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Why is 4P curve fit curve recommended for ELISA data analysis and where can I find access to this data analysis software?
For the most accurate results, we normally recommend to use a 4-parameter logistic (4-PL) curve fitting software, as 4-PL fitting…
Tue, Jul 18, 2023
What is the best method to store PROTEOSTAT® Aggresome Detection Reagent in ENZ-51023, ENZ-51035 and ENZ-51027?
It is best to freeze PROTEOSTAT® Protein Detection reagent at -20° for long term storage. When many experiments are planned for following…
Tue, Jul 18, 2023
What precautions do we recommend while working with PROTEOSTAT® Aggresome Detection Reagent in ENZ-51023, ENZ-51035 and ENZ-51027?
The PROTEOSTAT® Detection Reagent in all these references (ENZ-51023, ENZ-51035 and ENZ-51027) is light sensitive. Avoid direct exposure of the…
Tue, Jul 18, 2023
How long can anti-DNP biotin antibody and streptavidin-HRP be stored once reconstituted for use with Protein Carbonyl ELISA, ALX-850-312?
Anti-DNP-biotin antibody (G) can be stored at -20 °C, for 1-2 weeks and streptavidin-horseradish peroxidase (H) can also be stored…
Tue, Jul 18, 2023
Can you provide an overview of steps in Intracellular Calcium mobilization assay with FLUOFORTE® Calcium assay kit, ENZ-51016 ?
The main steps for the FLUOFORTE® Calcium assay kit (ENZ51016) involves loading cells with dye that fluoresce when exposed to…
Tue, Jul 18, 2023
How does EFLUXX-ID® Green multidrug resistance assay kit, ENZ-51029 and EFLUXX-ID® Gold multidrug resistance assay kit, ENZ-51030 detect activity of transporter proteins?
EFLUXX-ID® Green and Gold Dyes in ENZ-51029 and ENZ-51030 kits are proprietary AM-esters. EFLUXX-ID® dye is a hydrophobic non-fluorescent compound…
Tue, Jul 18, 2023
How do MITO-ID® Red detection (ENZ-51007) and MITO-ID® Green Detection kit (ENZ-51022) work?
MITO-ID® Red detection (ENZ-51007) and MITO-ID® Green Detection kit (ENZ-51002) appear to target the inner mitochondrial membrane in mammalian cells, which…
Tue, Jul 18, 2023
Would COX activity assay kit, ADI-907-003 work with human plasma and mouse cell lysate samples?
COX activity assay kit, ADI-907-003 https://www.enzolifesciences.com/fileadmin/files/manual/ADI-907-003_insert.pdf is designed for the quantitative determination of COX activity in biological samples. However, we…
Tue, Jul 18, 2023
What starting volume is required for COX activity assay kit, ADI-907-003?
For COX activity assay kit, ADI-907-003 we recommend starting volumes of 50 µL COX-I or COX-II preparations. Actual sample volumes required…
Tue, Jul 18, 2023
Does BIOARRAY™ Single-round RNA amplification and biotin labeling system, ENZ-42420 and ENZ-42421 have all the items necessary for amplification and labeling?
This kit, provided in 2 sizes (10 reactions: ENZ-42420; 100 reactions: ENZ-42421) contains all the buffers, enzymes, purification columns, elution…
Tue, Jul 18, 2023