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ELISA Immunoassays
For Quantitation Limit (QL) with E. coli Host Cell Protein ELISA kit, ENZ-KIT127, why is 10 ng/mL chosen for precision and accuracy?
A measurable slope is observed between 10 and 30 ng/mL concentrations and anything below 10 ng/mL cannot be easily validated.
Tue, Jul 18, 2023
What is the percent error range set for newly manufactured lots of E. coli Host Cell Protein ELISA kit, ENZ-KIT127?
We do not have defined cut-offs for % error when looking at the performance of new plate lots. Quality control…
Tue, Jul 18, 2023
Why is boiling samples necessary with Protein A ELISA kit, ADI-900-057?
Samples containing IgG requires boiling to dissociate Protein A in the sample from IgG
Tue, Jul 18, 2023
What is the recommended sample volume to use for the TFA extraction protocol with Substance P ELISA kit, ADI-900-018A ?
Frequently used sample volumes for Substance P ELISA kit range from 250 µl – 5ml. Depending on the type of…
Tue, Jul 18, 2023
How long does it take to dry down samples after blood is mixed with ice cold ethanol for Bradykinin ELISA, ADI-900-206?
Precise time to dry down depends on the efficiency of the SpeedVac. Usually it would take 2-4 hrs to completely…
Tue, Jul 18, 2023
At which step should the PMSF and Protease Inhibitor cocktail be added to the sample for Bradykinin ELISA kit, ADI-900-206?
As indicated in page 5 of user manual (Step 4), protease inhibitor cocktail and PMSF should be added during the…
Tue, Jul 18, 2023
Which proteasome subunit does the capture antibody in Proteasome ELISA kit, BML-PW0575 bind to?
Proteasome ELISA Kit, BML-PW0575 is an immunometric sandwich ELISA. The capture antibody is Alpha 6 subunit, 20S proteasome, mouse monoclonal…
Tue, Jul 18, 2023
Can the results form PEGylated protein ELISA kit, ADI-900-213 be used interpret as potential source of allergy to Polyethylene Glycol?
Enzo PEGylated protein ELISA kit, ADI-900-213-0001 is designated for research use only (RUO) and can not be used in diagnostics.
Tue, Jul 18, 2023
Do we have a protocol to lyse yeast cells to quantify intracellular cAMP concentrations with Direct cAMP ELISA kit, ADI-900-066A?
We provide 0.1 M HCl to lyse cells and tissue. We do not have a protocol for lysing yeast cells.
Tue, Jul 18, 2023
If a speed vac is not available to dry down samples, how can samples be dried while working with Bradykinin ELISA kit, ADI-900-206?
Vacuum centrifugation (e.g. SpeedVac) is usually the preferred option when a large number of samples has to be dried. In…
Tue, Jul 18, 2023
