Home Basic Research Technology Platforms Tissue Analysis Cytogenetics – FISH DEEPSEE® FISH Probes

DEEPSEE® FISH Probes

Simpler protocol. Same Day results.

Ready-to-Use FISH Probes

2-hour

Hybridization

No new equipment required

24–48 hr

Delivery

80 ready-to-use, directly labeled FISH probes for detection of chromosomal copy number changes, gene amplifications, deletions, translocations, and rearrangements across hematologic and solid tumor applications.

For Research Use Only (RUO)

Why DEEPSEE®

Built for labs that can’t wait until tomorrow

Every DEEPSEE^® probe is designed around one idea: a result shouldn’t take longer to produce than the question that prompted it. From a single vendor, across 80 probes, DEEPSEE^® removes the steps that slow labs down — without asking you to change how you work.

2-hour hybridization

Complete hybridization in 2 hours, not the usual overnight 12–16. Start in the morning, get results by afternoon.

Directly labeled

Pre-diluted, ready-to-use probes need no secondary labeling. Apply, hybridize, wash, mount.

24–48 hr delivery

Manufactured under rigorous QC, so a stockout never stalls a case.

80 probes, one vendor

Hematologic malignancies, solid tumors, and chromosomal controls, all from a single validation partner.

Bright, intense signals

High-contrast Orange and Green dyes, plus SEEBRIGHT^® DAPI mounting medium, for sharp signals that resist photobleaching.

Instrument compatible

Runs on HYBrite™, ThermoBrite^®, or manual protocols. No new equipment required.

How it works

A simplified workflow for same-day results

A streamlined 4-reagent, 5-step process carries a sample from slide to scored signal in about three hours. A single 2-hour hybridization step replaces the overnight incubation required by traditional FISH protocols.

Hybridization time, to scale Hours from probe application to score-ready signal

16 hr

Traditional FISHovernight incubation

12–16 hrs

DEEPSEE®start morning, read by afternoon

2 hrs

Slide prep

Deparaffinize & pretreat sample.

~30 min

Probe application

Apply ready-to-use probe, seal slide.

~5 min

Hybridization

37°C, no overnight incubation.

2 hours

Wash & mount

Post-hyb wash, DAPI mounting medium.

~20 min

Fluorescence analysis

Visualize & score signals.

Same day

~3 Hours Total: hands-on + hybridization time Start in the morning — analyze results before end of day

Complete probe catalog

A representative view of the DEEPSEE^® line

Hematologic malignancies

Catalog #	Probe name	Locus / target	Research significance
ENZ-GEN443	MYC Break Apart Dual Probe	8q24 (MYC)	Burkitt Lymphoma, DLBCL. Identifies MYC rearrangements; separation of Orange/Green signals indicates break-apart event at 8q24.
ENZ-GEN447	CCND1/IGH Split Extended FISH Probe	11q13 (CCND1) / 14q32 (IGH)	Mantle Cell Lymphoma. Detects t(11;14) translocation; CCND1/IGH fusion is a hallmark of mantle cell lymphoma.
ENZ-GEN448	BCL6 Break Apart FISH Probe	3q27 (BCL6)	DLBCL, Follicular Lymphoma. BCL6 rearrangements associate with aggressive B-cell lymphomas.
ENZ-GEN450	IGH/BCL2 Split FISH Probe	14q32 (IGH) / 18q21 (BCL2)	Follicular Lymphoma. Detects t(14;18), the most common FL translocation, upregulating BCL2.
ENZ-GEN451	D13S319/LAMP1 FISH Probe	13q14 (D13S319) / 13q34 (LAMP1)	CLL. Del(13q14) is the most frequent cytogenetic abnormality in CLL; favorable prognostic marker.
ENZ-GEN452	IGH Break Apart FISH Probe	14q32 (IGH)	Multiple Myeloma, B-cell Lymphoma. Screens for any rearrangement at 14q32.
ENZ-GEN454	BCL2 Break Apart FISH Probe	18q21 (BCL2)	Follicular Lymphoma, DLBCL. t(14;18) is a defining feature of follicular lymphoma.
ENZ-GEN457	BCR/ABL1 FISH Probe	22q11 (BCR) / 9q34 (ABL1)	CML, ALL (Ph+ leukemia). Detects the Philadelphia chromosome t(9;22); primary target of TKI therapy (imatinib).
ENZ-GEN458	D13S319/LAMP1/CEN12 FISH Probe	13q14 / 13q34 / CEN12	CLL Panel. Tri-locus probe for simultaneous assessment of 13q14 deletion with internal CEN12 reference.
ENZ-GEN460	ETV6 Break Apart FISH Probe	12p13 (ETV6)	ALL, AML, MDS. t(12;21) ETV6/RUNX1 is most frequent translocation in childhood B-ALL.
ENZ-GEN462	ATM/TP53 Extended FISH Probe	11q22 (ATM) / 17p13 (TP53)	CLL, Mantle Cell Lymphoma. High-risk cytogenetic markers associated with poor prognosis and chemoresistance.
ENZ-GEN463	NUP98 Break Apart Extended FISH Probe	11p15 (NUP98)	AML, ALL, MDS. Found in ~3% of AML; over 30 fusion partners identified.
ENZ-GEN465	CRLF2 Break Apart FISH Probe	Xp22.3/Yp11.3 (CRLF2)	B-cell ALL (Ph-like). Present in ~5–7% of B-ALL; associated with JAK pathway activation.
ENZ-GEN467	KMT2A Break Apart Extended FISH Probe	11q23 (KMT2A/MLL)	AML, ALL, MDS, infant leukemia. Over 80 translocation partners; hallmark of infant ALL.
ENZ-GEN468	MYC/IGH Split FISH Probe	8q24 (MYC) / 14q32 (IGH)	Burkitt Lymphoma, DLBCL (Double Hit). Detects t(8;14); the defining event of Burkitt lymphoma.
ENZ-GEN470	PML/RARA Extended FISH Probe	15q24 (PML) / 17q21 (RARA)	Acute Promyelocytic Leukemia (APL). Confirms ATRA/arsenic trioxide therapy eligibility.
ENZ-GEN472	ABL1 Break Apart FISH Probe	9q34 (ABL1)	CML, Ph+ ALL. Also detects NUP214/ABL1 and other ABL1 fusion partners in T-ALL.
ENZ-GEN473	RUNX1T1/RUNX1 Extended FISH Probe	8q22 (RUNX1T1) / 21q22 (RUNX1)	AML t(8;21). Core-binding factor AML with generally favorable prognosis.
ENZ-GEN477	CBFB Break Apart FISH Probe	16q22 (CBFB)	AML inv(16)/t(16;16). Identifies CBFB/MYH11 fusion associated with favorable prognosis.
ENZ-GEN478	IGH/MAFB Split Extended FISH Probe	14q32 (IGH) / 20q12 (MAFB)	Multiple Myeloma. t(14;20) MAFB rearrangement associated with adverse outcome.
ENZ-GEN484	ABL2 Break Apart FISH Probe	1q25 (ABL2)	ALL, AML. ETV6/ABL2 fusion found in imatinib-sensitive cases of chronic eosinophilic leukemia and ALL.
ENZ-GEN486	BIRC3/MALT1 FISH Probe	11q22.1 (BIRC3) / 18q21.32 (MALT1)	MALT Lymphoma, CLL. t(11;18) is the most common translocation in MALT lymphoma.
ENZ-GEN491	FGFR3/IGH Split FISH Probe	4p16 (FGFR3) / 14q32 (IGH)	Multiple Myeloma. t(4;14) in ~15% of myeloma; targetable with FGFR inhibitors.
ENZ-GEN492	11Q Dual Color/CEN11 FISH Probe	11q22–11q23 / CEN11	CLL, MDS. Detects 11q gain and deletion; 11q deletion includes ATM locus.
ENZ-GEN493	DUSP22/IRF4 Break Apart FISH Probe	6p25.3 (DUSP22) / 6p25.3 (IRF4)	ALK-negative ALCL. Distinguishes favorable- vs adverse-prognosis subsets.
ENZ-GEN496	IGH/MAF Split FISH Probe	14q32 (IGH) / 16q23 (MAF)	Multiple Myeloma. t(14;16) associated with high-risk disease.
ENZ-GEN528	MALT1 Break Apart FISH Probe	18q21 (MALT1)	MALT Lymphoma. Screens for any MALT1 rearrangement including t(14;18) and t(11;18).
ENZ-GEN536	ETV6/RUNX1 FISH Probe	12p13 (ETV6) / 21q22 (RUNX1)	Pediatric B-ALL. Most common chromosomal translocation in childhood ALL (~25%).
ENZ-GEN539	GATA2/MECOM Extended FISH Probe	3q21 (GATA2) / 3q26 (MECOM/EVI1)	MDS, AML. Defines a high-risk AML subtype.
ENZ-GEN549	ERG Break Apart FISH Probe	21q22 (ERG)	AML, ALL, Prostate Cancer. Also detects TMPRSS2/ERG fusion in prostate cancer.
ENZ-GEN553	ZNF384 Break Apart FISH Probe	12p13 (ZNF384)	B-cell ALL. Defines a distinct subtype (~5%); generally intermediate prognosis.
ENZ-GEN555	PBX1/TCF3 FISH Probe	1q23 (PBX1) / 19p13 (TCF3)	B-cell ALL. t(1;19) found in ~6% of pediatric B-ALL; favorable with intensified therapy.
ENZ-GEN449	TPRG1L/ABL2 FISH Probe	1p36 (TPRG1L) / 1q25 (ABL2)	1p/19q Glioma codeletion / Ph-like ALL. Dual-utility probe for CNS and hematologic applications.
ENZ-GEN453	Del 20p20q FISH Probe	20p11.21 (Control 20) / 20q12 (PTPRT)	MDS, AML, MPN. Isolated del(20q) confers favorable prognosis in MDS.
ENZ-GEN455	5p5q FISH Probe	5p15.31 (TAS2R1) / 5q31.2 (EGR1)	MDS (5q syndrome), AML. Isolated del(5q) defines 5q-syndrome responsive to lenalidomide.

Solid tumors

Catalog #	Probe name	Locus / target	Research significance
ENZ-GEN541	ERBB2 (HER2)/CEN17 FISH Probe	17q12 (ERBB2/HER2) / CEN17	Breast, Gastric Cancer. HER2/CEN17 ratio guides trastuzumab/pertuzumab/T-DM1 therapy selection.
ENZ-GEN444	MDM2/CEN12 Dual Probe	12q15 (MDM2) / CEN12	Liposarcoma, Glioblastoma, Osteosarcoma. Hallmark of well-differentiated/dedifferentiated liposarcoma.
ENZ-GEN445	TP53/CEN17 FISH Probe	17p13 (TP53) / CEN17	Multiple Cancers. TP53 deletion is the most common alteration in human cancer.
ENZ-GEN446	MET/CEN7 FISH Probe	7q31 (MET) / CEN7	NSCLC, Gastric Cancer. MET amplification targetable with capmatinib, tepotinib.
ENZ-GEN456	USP6 Break Apart FISH Probe	17p13 (USP6)	Aneurysmal Bone Cyst, Nodular Fasciitis. Pathognomonic for these benign lesions.
ENZ-GEN459	TFE3 Break Apart FISH Probe	Xp11.2 (TFE3)	Renal Cell Carcinoma (Xp11 translocation RCC), Alveolar Soft Part Sarcoma.
ENZ-GEN461	ROS1 Break Apart FISH Probe	6q22 (ROS1)	NSCLC, Cholangiocarcinoma. Sensitive to ROS1 inhibitors (crizotinib, entrectinib).
ENZ-GEN464	MAML2 Break Apart FISH Probe	11q21 (MAML2)	Mucoepidermoid Carcinoma. Positive cases have favorable prognosis vs. MAML2-negative.
ENZ-GEN466	NRG1 Break Apart FISH Probe	8p12 (NRG1)	NSCLC, Breast, Pancreatic Cancer. Targetable with afatinib and zenocutuzumab.
ENZ-GEN469	RET Break Apart FISH Probe	10q11.2 (RET)	Papillary Thyroid Cancer, NSCLC. Selpercatinib and pralsetinib are RET-specific approved inhibitors.
ENZ-GEN475	FGFR1 Break Apart FISH Probe	8p12 (FGFR1)	NSCLC, Bladder Cancer, Myeloid/Lymphoid Neoplasms. Defines 8p11 myeloproliferative syndrome.
ENZ-GEN479	RREB1/MYB/CEN6 FISH Probe	6p25 (RREB1) / 6q23 (MYB) / CEN6	Melanoma. Distinguishes melanoma from Spitz nevi.
ENZ-GEN480	FGFR1/CEN8 FISH Probe	8p12 (FGFR1) / CEN8	Breast, Lung, Bladder Cancer. Amplification in ~10% breast cancer squamous subtype.
ENZ-GEN481	NUTM1 Break Apart FISH Probe	15q14 (NUTM1)	NUT Carcinoma. Aggressive midline carcinoma in young patients.
ENZ-GEN482	JAZF1 Break Apart FISH Probe	7p15 (JAZF1)	Endometrial Stromal Sarcoma (Low-grade). JAZF1/SUZ12 t(7;17) in ~45% of low-grade ESS.
ENZ-GEN483	MYB Break Apart FISH Probe	6q23 (MYB)	Adenoid Cystic Carcinoma, Diffuse Astrocytoma. MYB/NFIB fusion in >85% of adenoid cystic carcinomas.
ENZ-GEN485	PHF1 Break Apart FISH Probe	6p21 (PHF1)	Endometrial Stromal Sarcoma, Ossifying Fibromyxoid Tumor.
ENZ-GEN487	CSF1R Break Apart FISH Probe	5q32 (CSF1R)	Tenosynovial Giant Cell Tumor (TGCT/PVNS). Pexidartinib is FDA-approved for TGCT.
ENZ-GEN488	YWHAE Break Apart FISH Probe	17p13 (YWHAE)	High-grade Endometrial Stromal Sarcoma. More aggressive than JAZF1-rearranged low-grade ESS.
ENZ-GEN489	NR4A3 Break Apart FISH Probe	9q22 (NR4A3)	Extraskeletal Myxoid Chondrosarcoma. Pathognomonic rearrangements.
ENZ-GEN490	COL1A1/PDGFB Dual Color FISH Probe	17q21 (COL1A1) / 22q13 (PDGFB)	Dermatofibrosarcoma Protuberans (DFSP). Positive cases are imatinib-responsive.
ENZ-GEN494	CDK4/CEN12 FISH Probe	12q14 (CDK4) / CEN12	Liposarcoma, Glioblastoma. Co-occurs with MDM2 amplification.
ENZ-GEN495	FGFR2/CEN10 FISH Probe	10q26 (FGFR2) / CEN10	Endometrial Cancer, Gastric Cancer, Cholangiocarcinoma. Targetable with erdafitinib, infigratinib, pemigatinib.
ENZ-GEN498	PDGFB Break Apart FISH Probe	22q13 (PDGFB)	DFSP. Complement to COL1A1/PDGFB dual-color fusion probe for complete characterization.
ENZ-GEN499	COL1A1 Break Apart FISH Probe	17q21 (COL1A1)	DFSP. Alternative to the fusion probe for confirmation.
ENZ-GEN524	DDIT3 Break Apart FISH Probe	12q13 (DDIT3/CHOP)	Myxoid/Round Cell Liposarcoma. FUS/DDIT3 and EWSR1/DDIT3 fusions are pathognomonic.
ENZ-GEN525	EWSR1 Break Apart FISH Probe	22q12 (EWSR1)	Ewing Sarcoma, Clear Cell Sarcoma, Desmoplastic Small Round Cell Tumor. Broad screening utility across >20 tumor types.
ENZ-GEN526	MYCN/CEN2 FISH Probe	2p24 (MYCN) / 2q11 (CEN2)	Neuroblastoma, Medulloblastoma, SCLC. MYCN/CEN2 ratio ≥4 defines amplification.
ENZ-GEN530	MYB/CEN6 FISH Probe	6q23 (MYB) / CEN6	Adenoid Cystic Carcinoma, Diffuse Astrocytoma (pediatric). MYB copy number assessment.
ENZ-GEN531	FUS Break Apart FISH Probe	16p11 (FUS)	Myxoid Liposarcoma, Low-grade Fibromyxoid Sarcoma.
ENZ-GEN533	MYC/CEN8 FISH Probe	8q24 (MYC) / CEN8	Multiple Cancers (Breast, Lung, Gastric). Ratio to CEN8 identifies high-level amplification.
ENZ-GEN537	NTRK1 Break Apart FISH Probe	1q23 (NTRK1)	Pan-cancer TRK Fusion. Larotrectinib and entrectinib have pan-cancer approval for NTRK-fusion tumors.
ENZ-GEN538	19p19q / Glioma FISH Probe	1p36 / 19q13 (Glioma 1p/19q)	Oligodendroglioma. Co-deletion predicts chemosensitivity (PCV, temozolomide).
ENZ-GEN543	P16/CEN9 FISH Probe	9p21 (CDKN2A/P16) / CEN9	Multiple Cancers (Melanoma, Bladder, Lung, GBM). Key biomarker in melanoma and mesothelioma grading.
ENZ-GEN440	CDKN2A/CEN3/7/17 Quad Probe Assay	9p21.3 / CEN3 / CEN7 / CEN17	Bladder Cancer (Urothelial Carcinoma). Four targets in a single hybridization.
ENZ-GEN548	KRAS/CEN12 FISH Probe	12p12 (KRAS) / CEN12	NSCLC, Colorectal, Pancreatic Cancer. High copy number associated with poorer outcomes.
ENZ-GEN550	FOXO1 Break Apart FISH Probe	13q14 (FOXO1)	Alveolar Rhabdomyosarcoma (ARMS). Distinguishes alveolar from embryonal RMS with prognostic implications.
ENZ-GEN552	FGFR2 Break Apart FISH Probe	10q26 (FGFR2)	Cholangiocarcinoma, Endometrial, Gastric Cancer. Pemigatinib is FDA-approved for FGFR2 fusion CCA.

Chromosomal control & enumeration probes

Catalog #	Probe name	Locus / target	Research significance
ENZ-GEN471	Chromosome 9 Control FISH Probe	CEN 9 (9p11-9q11)	Centromere 9 enumeration; reference control for chr9 copy number.
ENZ-GEN476	Chromosome 8 Control FISH Probe	CEN 8 (8p11.1-8q11.1)	Centromere 8 enumeration; reference for MYC/CEN8, FGFR1/CEN8 probes.
ENZ-GEN497	Chromosome 13/18/21 Control FISH Probe	SPEC 13 / CEN 18 / SPEC 21	Tri-probe panel for simultaneous detection of trisomies 13, 18, and 21.
ENZ-GEN529	Chromosome 03 Control Probe	CEN 3 (3p11-3q11)	Centromere 3 enumeration; internal reference for chr3 copy number assessment.
ENZ-GEN534	Chromosomes X and Y Control Probe	CEN X / CEN Y	Sex chromosome enumeration; gender identification in HSC transplant chimerism, Turner syndrome research.
ENZ-GEN542	Chromosome X/Yq12 Control FISH Probe	CEN X / Yq12 heterochromatin	Enhanced sex chromosome assessment targeting Yq12 heterochromatin.
ENZ-GEN554	Chromosome 4/10/17 Control FISH Probe	4p11 / CEN 10 / CEN 17	Tri-chromosome panel for FSHD and neurological disorder chromosomal studies.

That’s our full line of 80 probes. Use the search bar above to find yours, or download the complete brochure.

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Technical reference

Technical specifications

Format & composition

Format	Pre-diluted hybridization solution
Labeling	Directly labeled (no secondary detection)
Buffer	Formamide-based hybridization buffer
Volume per test	10 µL per reaction
Dye system	Orange / Green (probe-dependent)

Sample compatibility

FFPE tissue sections
Cytological preparations
Metaphase chromosomes
Interphase nuclei
Bone marrow smears

Storage & delivery

Shipping	Dry ice
Short-term storage	2–8°C, protected from light
Long-term storage	−20°C
Regulatory status	RUO — not for use in diagnostic procedures
Delivery	24–48 hr

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Technical and Application Support

Questions about a probe, protocol, or your results? Our technical and application support team is ready to help.

Technical Support

Volume pricing and institutional accounts

High-throughput labs and institutional accounts can access tiered pricing, standing orders, and blanket purchase agreements.

Customer Support

Available

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Purity	≥93% (HPLC)

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