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How specific are colorimetric/fluorogenic substrates in Matrix Metalloproteinase (MMP) drug discovery kits?
Several MMPs cleave both BML-P277-9090 substrate (OMNIMMP™ RED fluorogenic substrate peptide), BML-P278-9090 substrate (MPP-3 Fluorogenic Substrate peptide) and colorimetric substrate…
Tue, Jul 18, 2023
27 ml Assay Buffer is provided in ADI-901-002, ADI-901-153A, ADI-901-173 and in several other 5 plate ELSIA kits. Would this amount of buffer be sufficient for all of the samples after preparing standards?
Assay buffer in 5x 96 plate kits (e.g. in ADI-901-002, ADI-901-153A, ADI-901-173) is provided as 10x Concentrate and this requires…
Tue, Jul 18, 2023
What is the recommended dilutions for plasma samples obtained from subjects treated with aspirin for TXB2 ELISA kit (ADI-900-002 and ADI-901-002)?
When the TBX2 ELISA kit (ADI-900-002 and ADI-901-002) was developed with pooled samples, 1:100 dilution worked. So, this is a…
Tue, Jul 18, 2023
Does Enzo Protein A ELISA kit, ADI-900-057, detect Protein A purified on MabSelect PrismA and MabSelect SuRe™ resins?
Protein A ELISA kit (ADI-900-057) detects ligand that bind to GE bound MabSelect SuRe™ resin. We have not determined the…
Tue, Jul 18, 2023
What is the difference between Z-VAD(OMe)-FMK (BML-P416) and Z-VAD-FMK (ALX-260-020)? Which Caspase inhibitor is best for cell based applications? Which inhibitor works in cell free assays?
Z-VAD(OMe)-FMK (BML-P416) has methyl ester group making this irreversible caspase inhibitor cell permeable and therefore is used in cell based…
Tue, Jul 18, 2023
Can PAP-AMC, Tyrosinase substrate, BML-EI405 function in live cell assay?
PAP-AMC, Tyrosinase substrate, BML-EI405 has been used in vitro assays. We do not have information if this substrate works efficiently…
Tue, Jul 18, 2023
What is the formulation of BIOMOL® Green (BML-AK111)?
The formulation of BIOMOL® (BML-AK111) Green is proprietary.
Tue, Jul 18, 2023
Is solvent extraction of samples required TXB2 ELISA kit (ADI-900-002 and ADI-901-002)?
With many ELISA kits, including TXB2 ELISA kit (ADI-900-002 and ADI-901-002), extraction is required if the levels of marker is…
Tue, Jul 18, 2023
Why is a big change in OD not noticed in an inhibitor screening assay with Tyrosinase (human), (recombinant) (His-tag), BML-SE535?
Tyrosinase reaction rates reaches maximal velocity in less than two minutes where an initial burst phase is followed by steady-state…
Tue, Jul 18, 2023
What specific tips are useful for substrate preparation with Tyrosinase (human), (recombinant) (His-tag), BML-SE535 assay?
When preparaing an assay with Tyrosinase (human), (recombinant) (His-tag), BML-SE535, please consider that Substrate-1, MBTH (3-methyl-2-benzothiazolinone hydrazone) is unstable. Make…
Tue, Jul 18, 2023