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Where do I find validation data for Protein A ELISA kit, ADI-900-057 since this is not listed on datasheet?
Description of Validation experiments and the data for Protein A ELISA kit (ADI-900-057) are listed in pages from 9-11 of…
Tue, Jul 18, 2023
Do custom wavelengths for the Absorbance 96 plate reader cost extra?
No, commonly used wavelength filters can be chosen at no extra cost. The vast majority of filter wavelengths and applications…
Tue, Jul 18, 2023
How can staining be improved with the PROTEOSTAT® Aggresome detection kit, ENZ-51035?
Optimize the dilution of the dye provided with PROTEOSTAT® Aggresome detection kit (ENZ-51035) to improve staining. Also incubationb time can…
Tue, Jul 18, 2023
How can photobleaching be minimized with PROTEOSTAT® Aggresome detection kit, ENZ-51035?
PROTEOSTAT® dye is light sensitive. Avoid direct exposure of the reagent to intense light. In a microscopy experiment with PROTEOSTAT®…
Tue, Jul 18, 2023
Is there a requirement for bridging when changing lots of E. coli host cell protein ELISA kit, ENZ-KIT127?
A bridging study is required if this is a part of defined or specified SOP for a facility or a…
Tue, Jul 18, 2023
Can Standards and Samples be derivatized on different days with Protein Carbonyl ELISA kit, ALX-850-312?
Standards, Controls and samples should be treated simultaneously with DNP on the same day in parallel to maintain consistency. When…
Tue, Jul 18, 2023
What precautions do we recommend while working with PROTEOSTAT® Aggresome Detection Reagent in ENZ-51023, ENZ-51035 and ENZ-51027?
The PROTEOSTAT® Detection Reagent in all these references (ENZ-51023, ENZ-51035 and ENZ-51027) is light sensitive. Avoid direct exposure of the…
Tue, Jul 18, 2023
Why do we suggest 96 -well black wall microplate, preferably with a clear bottom for PROTEOSTAT® Protein aggregation assay, ENZ-51023
Black side wall microplates would minimize spillover of RFU reads between the neighboring wells when analyzing data obtained with PROTEOSTAT®…
Tue, Jul 18, 2023
What is the best method to store PROTEOSTAT® Aggresome Detection Reagent in ENZ-51023, ENZ-51035 and ENZ-51027?
It is best to freeze PROTEOSTAT® Protein Detection reagent at -20° for long term storage. When many experiments are planned for following…
Tue, Jul 18, 2023
Can Excel be used to plot read outs from ELISA? How to calculate reads from ELISA using Excel?
Our recommendation is to analyze ELISA reads by 4P curve fit. Many plate readers do have the ability to perform…
Tue, Jul 18, 2023