HPV Resources

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HPV resources

HPV image gallery

Real results with Enzo HPV reagents. Choose an application, an instrument, a sample and a target to find the images you need. Each image shows its product reference and links to its product page.

Application
Instrument
Sample
Target

Cervical tissue stained with AMPIVIEW HPV High-Risk RNA probes; positive nuclei appear dark red.
ISHAMPIVIEW® HPV High-Risk RNA probes

Cervical tissue hybridized with the PATHO-GENE HPV 16/18/31/33/51 probe and developed with HRP-DAB; positive nuclei are brown.
ISHPATHO-GENE® HPV 16/18/31/33/51 probe

Cervical tissue stained with the DIGX HPV 6/11 probe on a Leica BOND III at 40X; positive nuclei are magenta.
ISHDIGX® HPV 6/11 probe (40X)

Cervical tissue stained with the DIGX HPV 6/11 probe on a Leica BOND III at 10X, showing strong staining and low background.
ISHDIGX® HPV 6/11 probe (10X)

Tissue stained with the AMPIVIEW HPV HR-15 RNA probes on a Ventana platform, showing a high-expressing sample.
ISHAMPIVIEW® HPV HR-15 probes

HPV-infected cervical tissue stained with AMPIVIEW HPV 6/11 RNA probes; positive cells are blue-green.
ISHAMPIVIEW® HPV 6/11 RNA probes

HPV-infected cervical tissue stained with AMPIVIEW HPV 16/18 RNA probes; positive nuclei are dark blue.
ISHAMPIVIEW® HPV 16/18 RNA probes

HPV-infected cervical tissue stained with AMPIVIEW HPV 31/33/51 RNA probes; positive cells are blue-green.
ISHAMPIVIEW® HPV 31/33/51 RNA probes

FFPE cervical tissue stained for p16 with ENZ-ABS377; the dysplastic epithelium shows strong brown staining.
IHCp16INK4a monoclonal antibody

Cervical tissue stained with the Ki-67 antibody on a Leica BOND III; proliferating basal cells are brown.
IHCKi-67 monoclonal antibody

Manual 3-plex multiplex staining: HPV RNA by in situ hybridization, p16 and Ki-67 protein by immunohistochemistry, with DAPI counterstain.
IF3-plex multiplex staining

  • Manual
  • HPV 16/18/31/33/51 RNA (ISH)
  • p16 protein (IHC)
  • Ki-67 protein (IHC)
  • DAPI

Posters

Scientific posters with results from our HPV probes and antibodies.

Poster: Characterizing the Spatial Biology of HPV Infection in Tissue with Multiplexing. Fluorescence multiplex of HPV High-Risk RNA, Ki-67 and p16 on cervical tissue.

EACR 2025 · Poster

Characterizing the Spatial Biology of HPV Infection in Tissue with Multiplexing

K. Tian, M. Mathieu, J. Coleman and P. Pande · Enzo Life Sciences

  • AMPIVIEW® HPV High-Risk RNA probes detect HPV 16, 18, 31, 33 and 51 (E2 and E6/E7 targets)
  • Fluorescence multiplex of HPV RNA ISH with Ki-67 and p16 IHC on one FFPE cervical tissue section
  • Spatial view of all three markers in a precancerous lesion (CIN1), with HPV-negative cervix and negative control probes

Product: AMPIVIEW® HPV High-Risk RNA probes

Samples tested

30 sample types and cohorts from peer-reviewed studies by independent research groups, each with the Enzo product that was used.

Sample tested Product reference Product name Study
SiHa cells (13 integrated HPV16 copies per cell) ENZ-GEN145, ENZ-GEN146, ENZ-GEN147, ENZ-GEN148, ENZ-GEN320 AMPIVIEW® LoopRNA™ (LRP) HPV probes —
C33A cells (HPV-negative control) ENZ-GEN145, ENZ-GEN146, ENZ-GEN147, ENZ-GEN148, ENZ-GEN320 AMPIVIEW® LoopRNA™ (LRP) HPV probes —
Cervical FFPE biopsies, CIN1 ENZ-GEN145, ENZ-GEN146, ENZ-GEN147, ENZ-GEN148, ENZ-GEN320 AMPIVIEW® LoopRNA™ (LRP) HPV probes —
Cervical carcinoma biopsies, strongly and weakly HPV-infected ENZ-GEN145, ENZ-GEN146, ENZ-GEN147, ENZ-GEN148, ENZ-GEN320 AMPIVIEW® LoopRNA™ (LRP) HPV probes —
ThinPrep liquid-based cytology: HPV-positive HSIL and HPV-negative samples ENZ-GEN145, ENZ-GEN146, ENZ-GEN147, ENZ-GEN148, ENZ-GEN320 AMPIVIEW® LoopRNA™ (LRP) HPV probes —
Clinical samples with Ct values up to 38.6 (compared with Cobas HPV PCR) ENZ-GEN145, ENZ-GEN146, ENZ-GEN147, ENZ-GEN148, ENZ-GEN320 AMPIVIEW® LoopRNA™ (LRP) HPV probes —
HPV16-infected cervical epithelium, single-cell transcriptomic validation ENZ-GEN145, ENZ-GEN146, ENZ-GEN147, ENZ-GEN148, ENZ-GEN320 AMPIVIEW® LoopRNA™ (LRP) HPV probes Bedard 2023
HPV-associated head and neck squamous cell carcinoma: tumor-infiltrating B-cell response ENZ-ABS377 p16INK4a monoclonal antibody Wieland 2021
Penile intraepithelial neoplasia (82 lesions, 64 patients): foreskin, glans, shaft ENZ-ABS377 p16INK4a monoclonal antibody —
Testicular Leydig cell tumors (42 FFPE) ENZ-ABS377 p16INK4a monoclonal antibody —
Oropharyngeal squamous cell carcinoma (59) ENZ-ABS377 p16INK4a monoclonal antibody —
Anal canal squamous cell carcinoma (113) ENZ-ABS377 p16INK4a monoclonal antibody —
Adenosquamous carcinoma: multi-site microdissected glandular and squamous foci ENZ-ABS377 p16INK4a monoclonal antibody Poulain 2026
HPV-associated oropharyngeal SCC and primary pulmonary SCC: paired cases ENZ-ABS377 p16INK4a monoclonal antibody Schulte 2020
Nodal nevi (51) and nodal metastatic melanoma (56): 91 patients ENZ-ABS377 p16INK4a monoclonal antibody See 2020
Senescent corneal and conjunctival epithelial cells: cultured cells and Stevens-Johnson syndrome tissue ENZ-ABS377 p16INK4a monoclonal antibody Kitazawa 2023
Human abdominal subcutaneous white adipose tissue: 10 younger (30 years or less) and 10 older donors ENZ-ABS664 p16INK4a monoclonal antibody (Ready-to-Use) Whytock 2024
Intracranial C6 glioblastoma rat model: FFPE brain tissue after intranasal anti-miR-21 therapy ENZ-ABS678 Ki-67 monoclonal antibody Lee 2023
Oral squamous cell carcinoma (30) ENZ-32884 PATHO-GENE® HPV screening probe (HPV 6, 11, 16, 18, 31, 33, 51) Lajer 2011
Pharyngeal squamous cell carcinoma (19) ENZ-32884 PATHO-GENE® HPV screening probe (HPV 6, 11, 16, 18, 31, 33, 51) Lajer 2011
Oral controls (17) ENZ-32884 PATHO-GENE® HPV screening probe (HPV 6, 11, 16, 18, 31, 33, 51) Lajer 2011
Pharyngeal controls (22) ENZ-32884 PATHO-GENE® HPV screening probe (HPV 6, 11, 16, 18, 31, 33, 51) Lajer 2011
Human cervical FFPE biopsies, CIN1 (compared with AMPIVIEW® LoopRNA™) ENZ-32882, ENZ-32884, ENZ-32885, ENZ-32886, ENZ-32887 PATHO-GENE® type-specific HPV DNA probes —
Cervical carcinoma cohort: extrachromosomal HPV episome amplification ENZ-32882, ENZ-32884, ENZ-32885, ENZ-32886, ENZ-32887 PATHO-GENE® type-specific HPV DNA probes Rossi 2023
HPV-positive head and neck tumors: intratumoral heterogeneity and HPV integration ENZ-32882, ENZ-32884, ENZ-32885, ENZ-32886, ENZ-32887 PATHO-GENE® type-specific HPV DNA probes Akagi 2023
Localized anal squamous cell carcinoma: tissue-anchored HPV ctDNA biomarker ENZ-32882, ENZ-32884, ENZ-32885, ENZ-32886, ENZ-32887 PATHO-GENE® type-specific HPV DNA probes Morris 2025
Metastatic anal canal squamous cell carcinoma: 72-patient cohort ENZ-32882, ENZ-32884, ENZ-32885, ENZ-32886, ENZ-32887 PATHO-GENE® type-specific HPV DNA probes Morris 2017
Hodgkin’s disease lymph node biopsies (54) ENZ-32882, ENZ-32884, ENZ-32885, ENZ-32886, ENZ-32887 PATHO-GENE® type-specific HPV DNA probes Brousset 1991
Non-Hodgkin’s lymphomas (45) ENZ-32882, ENZ-32884, ENZ-32885, ENZ-32886, ENZ-32887 PATHO-GENE® type-specific HPV DNA probes Brousset 1991
Nasopharyngeal carcinomas (5) ENZ-32882, ENZ-32884, ENZ-32885, ENZ-32886, ENZ-32887 PATHO-GENE® type-specific HPV DNA probes Brousset 1991

Instruments tested

Our HPV reagents are used on manual workflows and on the main automated instruments.

  • Leica BOND
  • Leica BOND-III
  • Leica BOND-RX
  • Leica BOND-MAX
  • Ventana BenchMark ULTRA
  • Dako PT Link
  • Dako EnVision
  • Manual CSA / CISH
  • Confocal IF
Instrument Product reference Product name How it was used
Leica BOND ENZ-GEN145, ENZ-GEN146, ENZ-GEN147, ENZ-GEN148, ENZ-GEN320 AMPIVIEW® LoopRNA™ (LRP) HPV probes Automated ISH
ENZ-ABS377 p16INK4a monoclonal antibody IHC at 1:100, pH 9 retrieval, 10 minutes
ENZ-32882, ENZ-32884, ENZ-32885, ENZ-32886, ENZ-32887 PATHO-GENE® type-specific HPV DNA probes Automated ISH on cervical biopsies
Leica BOND-III ENZ-GEN112 DIGX® HPV 6/11 probe ISH on cervical tissue at 10X and 40X
ENZ-ABS377 p16INK4a monoclonal antibody IHC on FFPE cervical tissue
ENZ-ABS678 Ki-67 monoclonal antibody IHC on cervical tissue
ENZ-KIT159, ENZ-KIT160 POLYVIEW® PLUS HRP-DAB detection kits Detection chemistry validated on automated platforms
Leica BOND-RX ENZ-ABS377 p16INK4a monoclonal antibody IHC at 1:100, 20-minute AR9640 retrieval
Leica BOND-MAX ENZ-ABS377 p16INK4a monoclonal antibody Automated IHC
Ventana BenchMark ULTRA ENZ-KIT159, ENZ-KIT160 POLYVIEW® PLUS HRP-DAB detection kits Detection chemistry validated on automated platforms
Dako PT Link ENZ-32884 PATHO-GENE® HPV screening probe (HPV 6, 11, 16, 18, 31, 33, 51) Companion p16 IHC in the study of oral and pharyngeal carcinomas
Dako EnVision ENZ-ABS377 p16INK4a monoclonal antibody Manual IHC with SignalStain DAB at 1:100
Manual workflows ENZ-GEN145, ENZ-GEN146, ENZ-GEN147, ENZ-GEN148, ENZ-GEN320 AMPIVIEW® LoopRNA™ (LRP) HPV probes Manual ISH, 5-hour protocol
ENZ-32882, ENZ-32884, ENZ-32885, ENZ-32886, ENZ-32887 PATHO-GENE® type-specific HPV DNA probes Manual ISH, routine paraffin workflow
ENZ-32884 PATHO-GENE® HPV screening probe (HPV 6, 11, 16, 18, 31, 33, 51) Manual ISH
ENZ-ABS664 p16INK4a monoclonal antibody (Ready-to-Use) Avidin-biotin peroxidase manual IHC
ENZ-ABS678 Ki-67 monoclonal antibody Manual immunofluorescence
Confocal IF ENZ-ABS377 p16INK4a monoclonal antibody Confocal immunofluorescence at 1:100

The full product × platform × specimen validation matrix, with a citation for each entry, is available on request. Ask our technical team.

Latest citations

The latest peer-reviewed publications, from 2025 and 2026, that use Enzo HPV reagents. More than 110 publications are listed on the product pages.

Poulain F, et al. Cell Reports 2026;45:117470

Multi-site microdissected glandular and squamous foci of adenosquamous carcinoma. University of Liège, GIGA-Cancer, Hôpital Européen Georges-Pompidou.

p16 antibody ENZ-ABS377

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For research use only. Not for use in diagnostic procedures. All product names, logos and brands are the property of their respective owners. © 2026 Enzo Life Sciences.