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Organelle Detection
Is it possible to multiplex MITO-ID® Membrane Potential Detection Kit, ENZ-51018 with NUCLEAR-ID® Blue/Red Cell Viability Reagent (GFP-CERTIFIED®), ENZ-53005?
MITO-ID/NUCLEAR-ID (ENZ-51018/ENZ-53005) multiplex assay have been multiplexed in a 3D spheroid cultures of iPSC-derived human hepatocytes (iCell Hepatocytes 2.0) assay…
Tue, Jul 18, 2023
How do MITO-ID® Red detection (ENZ-51007) and MITO-ID® Green Detection kit (ENZ-51022) work?
MITO-ID® Red detection (ENZ-51007) and MITO-ID® Green Detection kit (ENZ-51002) appear to target the inner mitochondrial membrane in mammalian cells, which…
Tue, Jul 18, 2023
How can background staining be minimized with ORGANELLE-ID-RGB® III assay kit, ENZ-51032?
When using ORGANELLE-ID-RGB® III assay kit (ENZ-51032) the cell medium could be a problem. Staining can be improved by…
Tue, Jul 18, 2023
What is the likely explanation for lack of staining cells with NUCLEAR-ID® Red DNA stain, ENZ-52406 for FACS application, when 250-fold and 500-fold dilution and incubation at 37°C for 30 minutes were tried?
It is important to check cell density and to ensure that cell concentration is 1 x 105 to 5 x…
Tue, Jul 18, 2023
What is the mechanism of the Golgi-ID™ Green assay kit
The Green detection reagent included in the kit ENZ-51028 is a fluorescent sphingolipid analog that is metabolized by cells and…
Tue, Jul 18, 2023
Can DAPI be used as counterstaining for the nucleus with the nucleolar green detection kit Nucleolar-ID® Green detection kit for microscopy ENZ-51009 ?
Hoechst 33342 has been shown to work well. DAPI and Hoechst have similar spectral characteristics and either of them should work…
Tue, Jul 18, 2023
Following staining with Mito-ID® Red detection kit, ENZ-51007, are the cells viable and is it possible to propagate cells?
The MITO-ID® Membrane potential dye (ENZ-51007) is not toxic to the cells at the concentrations and staining time recommended. Experiments demonstrated…
Tue, Jul 18, 2023
How long does the signal last with Mito-ID® Membrane potential cytotoxicity kit for microplates kit, ENZ-51019?
Time-course study of mitochondrial membrane potential changes with the Mito-ID® Membrane potential cytotoxicity kit for microplates kit, ENZ-51019 can be…
Tue, Jul 18, 2023
What is the application of the antifade mounting medium hardset (ENZ-53002, ENZ-53003) ?
The Antifade mounting medium hardeset can be used as a mouning medium fo fixed cells and tissues, stained with fluorescent…
Tue, Jul 18, 2023
Antifade mounting medium hardset (ENZ-53002, ENZ-53003) appears to be clumped. What is the recommendation to get this in liquid form?
Antifade mounting medium hardset (ENZ-53002, ENZ-53003) needs to be warmed at 37-42°C for about 20 minutes, then vortexedt, if clumps are…
Tue, Jul 18, 2023