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How critical is four minutes centrifugation at 13,800 x g following boiling and cooling samples at step 5 in sample handling with Protein A ELISA kit, ADI-900-057?
The recommended centrifugation is 4 min at 13,800 x g. Depending on your centrifuge, you may need to adjust time…
Tue, Jul 18, 2023
Does ONO-RS-082, BML-ST320 inhibit all isoforms of phospholipase A2?
Unfortunately, we have not characterized inhibition of all isoforms of phospholipase A2 by BML-ST320. BML-ST320 is a well characterized in the…
Tue, Jul 18, 2023
What is the difference between Prostaglandin E2 high sensitivity ELISA kit, ADI-930-001 and PGE2 ELISA kit, ADI-900-001?
The PGE2 high sensitivity ELISA kit with catalog number ADI-930-001 was specifically developed to quantify the levels of PGE2 in…
Tue, Jul 18, 2023
What is the size of ENZO PATHO-GENE® HPV probes and what regions do they target?
All of the PATHO-GENE® HPV probe we offer, vary in size from 100 bp to 1 Kb and the probe…
Tue, Jul 18, 2023
What buffer should be used to dilute standards, ATP and active PKC in ADI-EKS-420A, PKC Kinase Activity Assay Kit?
Only Kinase Assay Dilution Buffer (80-1486) provided with PKC kinase activity kit (ADI-EKS-420A) should be used to dilute ATP, Standards…
Tue, Jul 18, 2023
What could be the reasons for lower spike and recovery results with Protein A ELISA kit, ADI-900-057?
There are several reasons for lower spike and recovery return values with Protein A ELISA kit (ADI-900-057). One possible reason…
Tue, Jul 18, 2023
Does PACOCF3, BML-ST336 inhibit all isoforms of phospholipase A2 (PLA2)?
PACOCF3 (BML-ST336) inhibits of both Ca2+-dependent cytosolic cPLA2 (IC50=45 µM) and Ca2+-independent phospholipases A2 iPLA2 (IC50=3.8 µM). Unfortunately, we have…
Tue, Jul 18, 2023
Can CHO host cell protein standards (ENZ-PRT122) be used as internal control in Western Blot or as standards in CHO Host cell protein ELISA kit (ENZ-KIT128)?
CHO host cell protein standards (ENZ-PRT122) can be used in Western blot or in an ELISA. With CHO Host cell…
Tue, Jul 18, 2023
What is the recommended final concentration of Z-Gly-Gly-Leu-AMC, BML-ZW8505 in a protease assay?
Z-Gly-Gly-Leu-AMC is added to a final concentration of 200 µM for solution based enzyme assays. For cell based assays, concentration of…
Tue, Jul 18, 2023
Buffer salts in 10x PBS-T wash buffer in E. coli host cell protein ELISA kit, ENZ-KIT127 has precipitated upon storage at 4°C. Is this usual and how to dissolve the salts?
On occasions, buffer salts precipitate out of solution at 4°C. Warming the bottle containing buffer at 37°C and frequent vortexing would…
Tue, Jul 18, 2023