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At which step should the PMSF and Protease Inhibitor cocktail be added to the sample for Bradykinin ELISA kit, ADI-900-206?
As indicated in page 5 of user manual (Step 4), protease inhibitor cocktail and PMSF should be added during the…
Tue, Jul 18, 2023
How long does it take to dry down samples after blood is mixed with ice cold ethanol for Bradykinin ELISA, ADI-900-206?
Precise time to dry down depends on the efficiency of the SpeedVac. Usually it would take 2-4 hrs to completely…
Tue, Jul 18, 2023
Why do we recommend 2,500 fold dilution of MITO-ID® Red Detection reagent, ENZ-51007 for staining aldehyde fixed and detergent permeabilized cells, but 10,000 fold dilution of MITO-ID® Detection reagent for live cells?
In aldehyde-fixed and detergent permeabilized sample, MITO-ID® Red Detection reagent diffuses passively once the membrane potential is lost. Therefore, higher dye…
Tue, Jul 18, 2023
How critical is the requirement for volumes of the sample to be 0.5 ml after diluting with Assay Buffer 13 0.5 ml at step 5 with Protein A ELISA kit, ADI-900-057?
Keeping the volume of sample to no more than 0.5ml is to ensure the entire Protein A in the sample…
Tue, Jul 18, 2023
What buffer should be used to dilute standards, ATP and active PKC in ADI-EKS-420A, PKC Kinase Activity Assay Kit?
Only Kinase Assay Dilution Buffer (80-1486) provided with PKC kinase activity kit (ADI-EKS-420A) should be used to dilute ATP, Standards…
Tue, Jul 18, 2023
What is the recommended solvent to dissolve Microcystin-LR, ALX-350-012 and what is the maximum solubility?
Maximum solubility of Microcystin-LR (ALX-350-012) is 10 mg/ml in 100 % ethanol, methanol or DMSO.
Tue, Jul 18, 2023
Does ENZO PATHO-GENE® HPV probes target E1, L2, E6 and E7 regions in the HPV genome? What is known about the targeting region of HPV probes?
All of the PATHO-GENE® HPV probes we offer, vary in size from 100 bp to 1 Kb and the probes…
Tue, Jul 18, 2023
What is the size of ENZO PATHO-GENE® HPV probes and what regions do they target?
All of the PATHO-GENE® HPV probe we offer, vary in size from 100 bp to 1 Kb and the probe…
Tue, Jul 18, 2023
Can test compound dissolved in DMSO be used in cell based assay to measure Reactive Oxygen Species with ROS-ID® Total ROS/Superoxide detection kit, ENZ-51010
We cannot confirm if dissolving the compound in DMSO could affect the assay, since DMSO could inhibit hydroxyl radical generation…
Tue, Jul 18, 2023
What is the lot-to-lot variation for CHO host cell protein ELISA kit, ENZ-KIT128?
In general, the antibodies provided with CHO host cell protein ELISA kit (ENZ-KIT12)8 are stable and we do not change…
Tue, Jul 18, 2023