Do we have a protocol to lyse yeast cells to quantify intracellular cAMP concentrations with Direct cAMP ELISA kit, ADI-900-066A?
We provide 0.1 M HCl to lyse cells and tissue. We do not have a protocol for lysing yeast cells.
Tue, Jul 18, 2023
What is the difference between Leptin (mouse), (recombinant), ALX-201-035 and Leptin (mouse), (recombinant), BML-SE160?
Leptin (mouse), (recombinant), ALX-201-035 is tested for and certified as endotoxin free
Tue, Jul 18, 2023
If a speed vac is not available to dry down samples, how can samples be dried while working with Bradykinin ELISA kit, ADI-900-206?
Vacuum centrifugation (e.g. SpeedVac) is usually the preferred option when a large number of samples has to be dried. In…
Tue, Jul 18, 2023
What is the difference between Prostaglandin E2 high sensitivity ELISA kit, ADI-930-001 and PGE2 ELISA kit, ADI-900-001?
The PGE2 high sensitivity ELISA kit with catalog number ADI-930-001 was specifically developed to quantify the levels of PGE2 in…
Tue, Jul 18, 2023
Does ONO-RS-082, BML-ST320 inhibit all isoforms of phospholipase A2?
Unfortunately, we have not characterized inhibition of all isoforms of phospholipase A2 by BML-ST320. BML-ST320 is a well characterized in the…
Tue, Jul 18, 2023
How critical is the requirement for volumes of the sample to be 0.5 ml after diluting with Assay Buffer 13 0.5 ml at step 5 with Protein A ELISA kit, ADI-900-057?
Keeping the volume of sample to no more than 0.5ml is to ensure the entire Protein A in the sample…
Tue, Jul 18, 2023
How critical is four minutes centrifugation at 13,800 x g following boiling and cooling samples at step 5 in sample handling with Protein A ELISA kit, ADI-900-057?
The recommended centrifugation is 4 min at 13,800 x g. Depending on your centrifuge, you may need to adjust time…
Tue, Jul 18, 2023
What buffer should be used to dilute standards, ATP and active PKC in ADI-EKS-420A, PKC Kinase Activity Assay Kit?
Only Kinase Assay Dilution Buffer (80-1486) provided with PKC kinase activity kit (ADI-EKS-420A) should be used to dilute ATP, Standards…
Tue, Jul 18, 2023
What is the recommended solvent to dissolve Microcystin-LR, ALX-350-012 and what is the maximum solubility?
Maximum solubility of Microcystin-LR (ALX-350-012) is 10 mg/ml in 100 % ethanol, methanol or DMSO.
Tue, Jul 18, 2023
Does ENZO PATHO-GENE® HPV probes target E1, L2, E6 and E7 regions in the HPV genome? What is known about the targeting region of HPV probes?
All of the PATHO-GENE® HPV probes we offer, vary in size from 100 bp to 1 Kb and the probes…
Tue, Jul 18, 2023
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