Lab Essentials
AMPIVIEW® RNA probes
Enabling Your Projects 
GMP Services
Bulk Solutions
Research Travel Grant
Have You Published Using an Enzo Product?
Life Sciences Products
Do eicosanoid assays require an extraction?
The majority of samples do not require an extraction. However, in some situations, an extraction will be required when the…
Tue, Jul 18, 2023
What species can the eicosanoid ELISA kits be used for?
Eicosanoids are not species-specific. However, the antisera used in a particular assay may present an interference effect due to the…
Tue, Jul 18, 2023
What antibody pairs are used in the kit?
This varies from kit to kit. Refer to the product specific instruction manual for more information.
Tue, Jul 18, 2023
When using an eicosanoid ELISA kit, how much indomethacin should I use in my samples?
You should follow the suggestion in the product specific manual, and add up to 10µg per ml of liquid sample.…
Tue, Jul 18, 2023
What is the difference between the 8-iso-PGF<sub><span style="font-size: 10pt;">2a</span></sub> ELISA kit and the Direct 8-iso-PGF<sub><span style="font-size: 10pt;">2a</span></sub> ELISA kit?
The regular 8-iso-PGF2a kit is designed to measure free isoprostane in samples. Direct 8-iso-PGF2a measures total isoprostane levels, both free…
Tue, Jul 18, 2023
Can I measure urinary steroid levels in Enzo’s hormone ELISA kits?
Steroids are relatively insoluble and must be altered by being conjugated to glucoronic acid for elimination. The antibodies in our…
Tue, Jul 18, 2023
Do I need to add Steroid Displacement Reagent to my samples prior to analysis with my hormone ELISA kits?
If total steroid levels are to be measured, Steroid Displacement Reagent (SDR) should be added to the samples containing binding…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, can I use other Streptavidin-HRP solutions?
Yes, however the reagent should be optimized (titered) to assure acceptable signal intensity and background are achieved.
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, is it really necessary to add stop solution before reading the plate?
While most plate readers have the ability to read optical density in the blue spectrum, they are usually more sensitive…
Tue, Jul 18, 2023
How do I know whether to use the Nitric Oxide (NO<sub>2-</sub>/NO<sub>3-</sub>) detection kit or the Nitric Oxide (total) detection kit?
The regular kit measures both major metabolic products of NO — nitrate and nitrite. The Total kit measures these metabolites…
Tue, Jul 18, 2023