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When using an IMMUNOSET® ELISA development set, how can I avoid evaporation of the solutions while coating, blocking, and assaying?
Seal the plates with a plate sealer, plastic wrap, or foil during incubations.
Tue, Jul 18, 2023
What if my cells are not sufficiently lyzed for cAMP/cGMP quantification after the addition of 0.1M HCl?
Cell or tissue lysis can be enhanced by adding 0.1-1% Triton X-100 to 0.1M HCl prior to use. When used…
Tue, Jul 18, 2023
Which protein assay is compatible with lysates obtained with HCl treatment?
This question is actually better suited for a company supplying the protein assay you wish to use. Thermo, for example,…
Tue, Jul 18, 2023
Can I use my own lysis buffer for the preparation of my cell lysates prior to cAMP/cGMP quantification?
The kit has been optimized for using HCl to both lyse and dilute your standards and samples while used in…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, can I alter the volume or concentration of antibody for coating?
Altering the volume or concentration of the coating antibody will have an impact on assay performance. Users should validate the…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, will the type of plates I use for coating affect the assay performance?
We recommend using plates made with high binding plastic, similar to Corning plates (2592). The use of different types of…
Tue, Jul 18, 2023
My maximum binding for acetylated cAMP/cGMP standards and samples is only about 50%. Why?
This occurs if the standards and samples were acetylated but the B0 standard was not. Your data can still be…
Tue, Jul 18, 2023
I am not getting consistent results with my cAMP/cGMP quantification in cells. I think that my cells may be permeabilized.
For the most consistent results, we recommend that cells be fully disrupted before using the lysate in an assay. Disruption…
Tue, Jul 18, 2023
What protocol can be used for drying samples prior to cAMP/cGMP quantification?
Samples can be dried using either vacuum centrifugation (e.g. SpeedVac) or under a gentle stream of nitrogen or argon. For…
Tue, Jul 18, 2023