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After having measured the total glutathione content, is it possible to use the same samples on the plate so as to continue the procedure and measure the oxidized form of glutathione?
The measurements of total and oxidized glutathione are two completely different events; it is therefore not possible to use the…
Tue, Jul 18, 2023
Why and how should I treat my samples and the standard with 4-Vinylpyridine (4-VP)?
In order to measure oxidized glutathione (GSSG), you must eliminate GSH in the cell extract by treating with 4-VP, a…
Tue, Jul 18, 2023
What is the principle of the Glutathione (GSSG/GSH) assay?
The Glutathione (total), detection kit is a complete kit that allows the determination of total, reduced, and oxidized glutathione concentrations…
Tue, Jul 18, 2023
Will the cytokine ELISA kits from one species work with another species?
This will depend on the species homology. Human cytokine ELISA kits will generally not measure cytokines in other species. Mouse…
Tue, Jul 18, 2023
What is the purpose of treating samples with 5% metaphosphoric acid (MPA)?
MPA should be added to fresh samples prior to sample handling or sample storage. That is required to deproteinize samples…
Tue, Jul 18, 2023
Values for this assay are reported as pmol/well. How can I convert the values obtained by this kit into a value that is more commonly reported in the literature, such as pmol/L (pM)?
Since the volume of sample added to each well is 50µL, results can be converted to molarity by multiplying by…
Tue, Jul 18, 2023
When using an eicosanoid ELISA kit, how much indomethacin should I use in my samples?
You should follow the suggestion in the product specific manual, and add up to 10µg per ml of liquid sample.…
Tue, Jul 18, 2023
What is the difference between the 8-iso-PGF<sub><span style="font-size: 10pt;">2a</span></sub> ELISA kit and the Direct 8-iso-PGF<sub><span style="font-size: 10pt;">2a</span></sub> ELISA kit?
The regular 8-iso-PGF2a kit is designed to measure free isoprostane in samples. Direct 8-iso-PGF2a measures total isoprostane levels, both free…
Tue, Jul 18, 2023
Can I measure urinary steroid levels in Enzo’s hormone ELISA kits?
Steroids are relatively insoluble and must be altered by being conjugated to glucoronic acid for elimination. The antibodies in our…
Tue, Jul 18, 2023
Do I need to add Steroid Displacement Reagent to my samples prior to analysis with my hormone ELISA kits?
If total steroid levels are to be measured, Steroid Displacement Reagent (SDR) should be added to the samples containing binding…
Tue, Jul 18, 2023