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What can interfere with the assay?
Reducing agents (such as ascorbic acid, beta-mercaptoethanol, dithiothreitol, and cysteine) and thiol reactive compounds (such as maleimides) are known to…
Tue, Jul 18, 2023
My first assay for total glutathione worked well, but when I repeated the assay to test the oxidized samples, the curve was essentially flat-to-negative.
Oxidized glutathione is very difficult to detect because it is rapidly turned over and converted, so there is only a…
Tue, Jul 18, 2023
After having measured the total glutathione content, is it possible to use the same samples on the plate so as to continue the procedure and measure the oxidized form of glutathione?
The measurements of total and oxidized glutathione are two completely different events; it is therefore not possible to use the…
Tue, Jul 18, 2023
Why and how should I treat my samples and the standard with 4-Vinylpyridine (4-VP)?
In order to measure oxidized glutathione (GSSG), you must eliminate GSH in the cell extract by treating with 4-VP, a…
Tue, Jul 18, 2023
What is the principle of the Glutathione (GSSG/GSH) assay?
The Glutathione (total), detection kit is a complete kit that allows the determination of total, reduced, and oxidized glutathione concentrations…
Tue, Jul 18, 2023
Will the cytokine ELISA kits from one species work with another species?
This will depend on the species homology. Human cytokine ELISA kits will generally not measure cytokines in other species. Mouse…
Tue, Jul 18, 2023
Can I run cell lysates with the Nitric Oxide detection kits?
Yes. First remove the media from the cells. Pellet them and wash with PBS before lysing the cells in the…
Tue, Jul 18, 2023
My cell culture samples are in DMEM. Will this interfere with the Nitric Oxide assay?
Yes. The nitrate salt used in this particular media will cause interference. It is important to check the published media…
Tue, Jul 18, 2023
I am using the Direct 8-iso PGF<sub><span style="font-size: 10pt;">2a</span></sub> ELISA kit. After I add the HCl to neutralize my samples, it is not reading in the range of pH 6-8. What should I do?
At this point, you have added HCl to NaOH which will yield NaCl + H2O. There is little buffering capacity…
Tue, Jul 18, 2023
If I have 2N NaOH hydrolyzed samples for an 8-iso-PGF<sub><span style="font-size: 10pt;">2a</span></sub> determination, do I need to extract these samples?
No, the Direct 8-iso-PGF2a kit is specifically designed to help you obtain results as quickly and easily as possible. The…
Tue, Jul 18, 2023