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Can the Serotonin ELISA kit, ADI-900-175, be used with samples from any species?
Serotonin is conserved across all species and the antibody provided in the kit will bind to Serotonin independently from the…
Tue, Jul 18, 2023
Does the eFluxx-ID® Green multidrug resistance assay kit, ENZ-51029 and eFluxx-ID® Gold multidrug resistance assay kit, ENZ-51030 kits work with frozen samples?
The EFLUXX-ID® multidrug resistance assay kits (ENZ-51029, ENZ-51030) have been validated for live cell analysis. If cells are healthy upon…
Tue, Jul 18, 2023
Can a multiplex assay be set up for testing three pumps at a time with The EFLUXX-ID® multidrug resistance assay kits (ENZ-51029, ENZ-51030)?
It is not possible to differentiate the pumps in a single assay with the EFLUXX-ID® multidrug resistance assay kits (ENZ-51029,…
Tue, Jul 18, 2023
Can DAPI be used as counterstaining for the nucleus with the nucleolar green detection kit Nucleolar-ID® Green detection kit for microscopy ENZ-51009 ?
Hoechst 33342 has been shown to work well. DAPI and Hoechst have similar spectral characteristics and either of them should work…
Tue, Jul 18, 2023
Which type of plasma samples can be used with your ADI-900-020A, Endothelin-1 ELISA kit (Heparin, EDTA or Citrate)?
The kit has been validated using EDTA plasma samples. Heparin and citrate plasma were not tested. We generally recommend EDTA plasma for…
Tue, Jul 18, 2023
How essential is the addition of aprotinin when collecting blood samples?
The aprotinin is included to help prevent any degradation of ET-1, but also to prevent any further cleavage of the…
Tue, Jul 18, 2023
How long may plasma samples be stored (with EDTA and aprotinin or with EDTA-only)? Has this been tested? Is there some degradation of Endothelin-1 while samples are stored?
Plasma samples collected as directed with EDTA and Aprotinin will be stable for at least 1 month at -80°C without…
Tue, Jul 18, 2023
Is the kit specific to Endothelin-1?
The antibodies used in this ELISA are highly specific with 100% cross-reactivity to ET-1, 21% to ET-2, 3.6% to ET-3…
Tue, Jul 18, 2023
The sensitivity of ADI-900-020A is listed as 0.41 pg/mL, whereas the standard range only goes as low as 0.78 pg/mL. What is the limit of detection of the assay?
Limit of detection is a statistical measurement meant to indicate the lowest concentration that the assay can differentiate from zero…
Tue, Jul 18, 2023
Is BIO-PROBE® 3’-Oligonucleotide labeling kit with bio-16-ddUTP, ENZ-42730-31 compatible with EMSA?
BIOPROBE® 3′-Oligonucleotide labeling kit with bio-16-ddUTP, ENZ-42730-31can be used for gel shift assays since the reaction yields uniformly labeled product.
Tue, Jul 18, 2023