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How should I store reconstituted standard, antibody and conjugatefor ADI-900-175?
The standard, antibody and conjugate are single use items and should to be stored at -20°C. Any leftover of reconstituted…
Tue, Jul 18, 2023
Do you sell a positive control for ADI-900-175, Serotonin ELISA kit, or could you advice on a suitable one?
We do not provide positive control samples with the kit. If you need to ensure accurate recovery of Serotonin from…
Tue, Jul 18, 2023
How were the <i>E.coli</i> host cell protein standards generated?
Most of the E.coli strains used in the industry and in the production of biologics are derived from K12. The…
Tue, Jul 18, 2023
Why do you use protein A affinity columns to purify the antibodies included in ENZ-KIT127, ENZ-KIT128, and ENZ-KIT162 instead of HCP affinity columns?
The main reason is product consistency. Protein A affinity columns have been around for quite a while. They are well…
Tue, Jul 18, 2023
What is the half-life of Hypoxia Red Detection Reagent and Oxidative Stress Detection Reagent in the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042?
We have not determined the half-life of the dyes provided with the ROS-ID® Hypoxia/Oxidative stress detection kit (ENZ-51042) but both…
Tue, Jul 18, 2023
The sensitivity of ADI-900-020A is listed as 0.41 pg/mL, whereas the standard range only goes as low as 0.78 pg/mL. What is the limit of detection of the assay?
Limit of detection is a statistical measurement meant to indicate the lowest concentration that the assay can differentiate from zero…
Tue, Jul 18, 2023
Is the kit specific to Endothelin-1?
The antibodies used in this ELISA are highly specific with 100% cross-reactivity to ET-1, 21% to ET-2, 3.6% to ET-3…
Tue, Jul 18, 2023
How long may plasma samples be stored (with EDTA and aprotinin or with EDTA-only)? Has this been tested? Is there some degradation of Endothelin-1 while samples are stored?
Plasma samples collected as directed with EDTA and Aprotinin will be stable for at least 1 month at -80°C without…
Tue, Jul 18, 2023
How essential is the addition of aprotinin when collecting blood samples?
The aprotinin is included to help prevent any degradation of ET-1, but also to prevent any further cleavage of the…
Tue, Jul 18, 2023
Which type of plasma samples can be used with your ADI-900-020A, Endothelin-1 ELISA kit (Heparin, EDTA or Citrate)?
The kit has been validated using EDTA plasma samples. Heparin and citrate plasma were not tested. We generally recommend EDTA plasma for…
Tue, Jul 18, 2023