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What could be the reason for low response to Wnt induction with LEADING LIGHT® Wnt Reporter Assay, ENZ-61001?
If observing a low response to Wnt induction with LEADING LIGHT® Wnt Reporter Assay (ENZ-61001), please consider the following. The…
Tue, Jul 18, 2023
Is there a requirement for cell lysis with LEADING LIGHT® Wnt Reporter Assay, ENZ-61001
LEADING LIGHT® Wnt Reporter Assay (ENZ-61001) is a cell-based luciferase and does not require lysis and chemiluminescence signals can be…
Tue, Jul 18, 2023
Does ORGANELLE-ID-RGB® III assay kit, ENZ-51032, bind to a target protein in ER and Golgi compartment?
The Golgi-green dye in the ORGANELLE-ID-RGB® III assay kit (ENZ-51032) is a fluorescent lipid analog which is transported directly into…
Tue, Jul 18, 2023
Can PAP-AMC, Tyrosinase substrate, BML-EI405 function in live cell assay?
PAP-AMC, Tyrosinase substrate, BML-EI405 has been used in vitro assays. We do not have information if this substrate works efficiently…
Tue, Jul 18, 2023
How can I improve the intensity of the signal obtained with the LEADING LIGHT® Wnt reporter cells?
Conditions in terms of cell density, culture medium, and incubation have to be respected. It is very important that there…
Tue, Jul 18, 2023
Which instrument should I use with your LEADING LIGHT® Wnt reporter assay starter kit to measure Wnt activity?
A plate luminometer capable of reading glow chemiluminescence is required. The light emitted by our LEADING LIGHT® Wnt reporter cell…
Tue, Jul 18, 2023
How long should I incubate my LEADING LIGHT® Wnt reporter cells after adding the luciferase substrate?
Signals should be read 10 minutes after adding the luciferase substrate with a multichannel pipette. The signal is stable enough…
Tue, Jul 18, 2023
Why was Wnt3a chosen as agonist for the LEADING LIGHT® Wnt reporter cell line?
Wnt3a is the most commonly studied agonist of the canonical Wnt signaling pathway and that is why it was selected…
Tue, Jul 18, 2023
Can you share the recipe of the Cell Growth Medium?
This information is considered to be proprietary and we cannot disclose the actual recipe. It contains additives and nutrients. These…
Tue, Jul 18, 2023
Do I need to use the LEADING LIGHT® Wnt cell line medium pack?
The LEADING LIGHT® Wnt cell line medium pack (ENZ-60003) is made of a cell freezing medium, a cell growth medium…
Tue, Jul 18, 2023