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How can the high background be reduced?
During incubation with NBT/BCIP Reaction Mixture, localized blue-purple precipitate is formed. Decreasing the incubation time with NBT/BCIP Reaction Mixture can reduce the…
Tue, Jul 18, 2023
Can the Progesterone ELISA kit be used with samples from any species?
Progesterone is conserved across all species and the antibody provided in the kit will bind to progesterone independently from the…
Tue, Jul 18, 2023
How should I treat samples with the steroid displacement reagent (SDR) for analysis with the Testosterone ELISA kit?
The Steroid Displacement Reagent should be used to treat appropriate samples in the following manner: Add 1µl of SDR per…
Tue, Jul 18, 2023
Can the Testosterone ELISA kit be used with samples from any species?
Testosterone is conserved across all species and the antibody provided in the kit will bind to Testosterone independently from the…
Tue, Jul 18, 2023
Can I use the Testosterone ELISA kit with human plasma and serum samples?
Sample types that have been validated are indicated on our website in the field “Application” and in the kit insert.…
Tue, Jul 18, 2023
What is the positive control provided in the kit?
The positive control provided in the kit ADI-EKS-390A is recombinant human PKAc beta (or catalytic subunit C-beta of PKA) expressed…
Tue, Jul 18, 2023
Can I use my lysis buffer instead of the lysis buffer indicated in the kit insert?
The buffer suggested in the kit insert has been optimized by our development scientists. It is likely that an alternative buffer will work,…
Tue, Jul 18, 2023
Can I use this kit to measure the concentration of PKA in my samples?
The kit does not measure the concentration of PKA in samples. It is intended for looking at relative PKA kinase activity.
Tue, Jul 18, 2023
Do you check for cross-reactivity with other steroids?
Yes, cross-reactivities for a number of related steroid compounds were determined and are listed in the kit insert.
Tue, Jul 18, 2023
Should I multiply the results obtained by the dilution factor?
Yes, the dilution factor must be applied to the calculated data to get the correct amount of biological marker present…
Tue, Jul 18, 2023