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What is the minimum sample volume for using the kit?
The assay requires 100 µL of diluted sample per well. The amount needed, therefore, depends on the samples themselves and…
Tue, Jul 18, 2023
If the test protein is a variant with near identical amino acid sequence with the exception of one residue, is one peak expected ?
There may be a possibility of different conformations of the same protein aggregating at different temperatures. The sample could have…
Tue, Jul 18, 2023
What thermal cyclers have the protocol been tested with ProteoStat® Thermal shift stability assay kit, ENZ-51027?
ProteoStat® Thermal shift stability assay should work on all types of thermal cyclers in the market. It has been tested…
Tue, Jul 18, 2023
Would membrane proteins work with ProteoStat® Thermal Shift Stability Assay Kit, ENZ-51027, and ProteoStat® Detection Reagent in ProteoStat® Protein Aggregation Assay, ENZ-51023?
We have not tested the ProteoStat® Protein Aggregation Assay, ENZ-51023, or ProteoStat® Thermal Shift Stability Assay Kit, ENZ-51027, with membrane…
Tue, Jul 18, 2023
Do you sell positive controls that can be run with your steroid ELISA kits?
We do not provide positive control samples with our kits. If you need to ensure accurate recovery of any analyte…
Tue, Jul 18, 2023
How can I overcome sample matrix interference?
The easiest way to overcome sample matrix interference is by diluting your sample with assay buffer. In order to determine…
Tue, Jul 18, 2023
How should I test for possible matrix interference with my samples?
Matrix interference describes the situation where the sample matrix (buffer salts, pH, additives like detergents or solvents, proteins, …) interferes…
Tue, Jul 18, 2023
Which is the best dilution for my sample type?
In the manual, section “Sample recoveries”, we provide a list of recommended dilutions. Those are the dilutions our development scientists…
Tue, Jul 18, 2023
Why is there sample dilution necessary in this ELISA Kit?
There are several reasons for dilutions. In some cases samples might read above the standard curve, thus requiring a dilution…
Tue, Jul 18, 2023
How does the steroid displacement reagent (SDR) work?
Steroids in circulation are almost all ionically bound to carrier proteins such as albumin or more specific binding proteins. Treating…
Tue, Jul 18, 2023