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Life Sciences
How should samples be stored after preparation for the NGAL (human) ELISA kit?
Samples should be aliquoted and stored at +2-8 °C for immediate analysis, or frozen at -20 °C for analysis within…
Tue, Jul 18, 2023
How should urine samples be prepared for use with the NGAL (human) ELISA kit?
Urine preparation usually only requires centrifugation if there is inflammation or infection of the urinary tract (to remove polymorphonuclear neutrophils…
Tue, Jul 18, 2023
How should plasma samples be prepared for use with the NGAL (human) ELISA kit?
Collect whole blood into commercially available anticoagulant-treated tubes e.g., EDTA-treated (lavender tops) or citrate-treated (light blue tops). Heparinized tubes (green…
Tue, Jul 18, 2023
The kit insert indicates that the PKA enzyme is provided as positive control. Is it possible to use it to perform a standard curve?
The active PKA kinase provided in the kit is to be used as a positive control and is not intended…
Tue, Jul 18, 2023
I am seeing well-to well variability amongst triplicates in the standards and samples. Values are within expected range but varies. What could be the reason?
Well-to well variability might be due to less than optimal shaking. Make sure a gentle but thorough mixing is applied as indicated…
Tue, Jul 18, 2023
How should I treat samples with the steroid displacement reagent (SDR) for analysis with the Progesterone ELISA kit?
The Steroid Displacement Reagent should be used to treat appropriate samples in the following manner: Add 1µl of SDR per…
Tue, Jul 18, 2023
Can the Progesterone ELISA kit be used with samples from any species?
Progesterone is conserved across all species and the antibody provided in the kit will bind to progesterone independently from the…
Tue, Jul 18, 2023
How should I treat samples with the steroid displacement reagent (SDR) for analysis with the Testosterone ELISA kit?
The Steroid Displacement Reagent should be used to treat appropriate samples in the following manner: Add 1µl of SDR per…
Tue, Jul 18, 2023
Can the Testosterone ELISA kit be used with samples from any species?
Testosterone is conserved across all species and the antibody provided in the kit will bind to Testosterone independently from the…
Tue, Jul 18, 2023
Can I use the Testosterone ELISA kit with human plasma and serum samples?
Sample types that have been validated are indicated on our website in the field “Application” and in the kit insert.…
Tue, Jul 18, 2023