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Life Sciences
Would COX activity assay kit, ADI-907-003 work with human plasma and mouse cell lysate samples?
COX activity assay kit, ADI-907-003 https://www.enzolifesciences.com/fileadmin/files/manual/ADI-907-003_insert.pdf is designed for the quantitative determination of COX activity in biological samples. However, we…
Tue, Jul 18, 2023
Can some guidance be provided to detect the activity of COX1 and COX2 from human plasma, and advise assay design with the Ibuprofen, meloxicam, known COX-2 inhibitors for COX activity assay kit, ADI-907-003?
We have not specifically validated this kit with human plasma samples. Page 5 of user manual provides the work flow. This provides…
Tue, Jul 18, 2023
What starting volume is required for COX activity assay kit, ADI-907-003?
For COX activity assay kit, ADI-907-003 we recommend starting volumes of 50 µL COX-I or COX-II preparations. Actual sample volumes required…
Tue, Jul 18, 2023
Does BIOARRAY™ Single-round RNA amplification and biotin labeling system, ENZ-42420 and ENZ-42421 have all the items necessary for amplification and labeling?
This kit, provided in 2 sizes (10 reactions: ENZ-42420; 100 reactions: ENZ-42421) contains all the buffers, enzymes, purification columns, elution…
Tue, Jul 18, 2023
Can gene-specific primers be used for cDNA synthesis with BIOARRAY™ Single-round RNA amplification and biotin labeling system, ENZ-42420 and ENZ-42421?
RNA amplification with BIOARRAYTM Single-round RNA amplification and biotin labeling system requires the use of primers included in the kit.…
Tue, Jul 18, 2023
How can background staining be minimized with ORGANELLE-ID-RGB® III assay kit, ENZ-51032?
When using ORGANELLE-ID-RGB® III assay kit (ENZ-51032) the cell medium could be a problem. Staining can be improved by…
Tue, Jul 18, 2023
What is the likely explanation for lack of staining cells with NUCLEAR-ID® Red DNA stain, ENZ-52406 for FACS application, when 250-fold and 500-fold dilution and incubation at 37°C for 30 minutes were tried?
It is important to check cell density and to ensure that cell concentration is 1 x 105 to 5 x…
Tue, Jul 18, 2023
Does 17ß-Estradiol ELISA kit, ADI-900-008 cross react with Estradiol 3-benzoate?
Estradiol 3-benzoate has not been determined for cross reactivity with17ß-Estradiol ELISA kit (ADI-900-008). The cross relativities for a number of…
Tue, Jul 18, 2023
27 ml Assay Buffer is provided in ADI-901-002, ADI-901-153A, ADI-901-173 and in several other 5 plate ELSIA kits. Would this amount of buffer be sufficient for all of the samples after preparing standards?
Assay buffer in 5x 96 plate kits (e.g. in ADI-901-002, ADI-901-153A, ADI-901-173) is provided as 10x Concentrate and this requires…
Tue, Jul 18, 2023
What is the recommended dilutions for plasma samples obtained from subjects treated with aspirin for TXB2 ELISA kit (ADI-900-002 and ADI-901-002)?
When the TBX2 ELISA kit (ADI-900-002 and ADI-901-002) was developed with pooled samples, 1:100 dilution worked. So, this is a…
Tue, Jul 18, 2023