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Life Sciences
What precautions do we recommend while working with PROTEOSTAT® Aggresome Detection Reagent in ENZ-51023, ENZ-51035 and ENZ-51027?
The PROTEOSTAT® Detection Reagent in all these references (ENZ-51023, ENZ-51035 and ENZ-51027) is light sensitive. Avoid direct exposure of the…
Tue, Jul 18, 2023
Why do we suggest 96 -well black wall microplate, preferably with a clear bottom for PROTEOSTAT® Protein aggregation assay, ENZ-51023
Black side wall microplates would minimize spillover of RFU reads between the neighboring wells when analyzing data obtained with PROTEOSTAT®…
Tue, Jul 18, 2023
What is the best method to store PROTEOSTAT® Aggresome Detection Reagent in ENZ-51023, ENZ-51035 and ENZ-51027?
It is best to freeze PROTEOSTAT® Protein Detection reagent at -20° for long term storage. When many experiments are planned for following…
Tue, Jul 18, 2023
Can Excel be used to plot read outs from ELISA? How to calculate reads from ELISA using Excel?
Our recommendation is to analyze ELISA reads by 4P curve fit. Many plate readers do have the ability to perform…
Tue, Jul 18, 2023
Why is 4P curve fit curve recommended for ELISA data analysis and where can I find access to this data analysis software?
For the most accurate results, we normally recommend to use a 4-parameter logistic (4-PL) curve fitting software, as 4-PL fitting…
Tue, Jul 18, 2023
How does EFLUXX-ID® Green multidrug resistance assay kit, ENZ-51029 and EFLUXX-ID® Gold multidrug resistance assay kit, ENZ-51030 detect activity of transporter proteins?
EFLUXX-ID® Green and Gold Dyes in ENZ-51029 and ENZ-51030 kits are proprietary AM-esters. EFLUXX-ID® dye is a hydrophobic non-fluorescent compound…
Tue, Jul 18, 2023
How do MITO-ID® Red detection (ENZ-51007) and MITO-ID® Green Detection kit (ENZ-51022) work?
MITO-ID® Red detection (ENZ-51007) and MITO-ID® Green Detection kit (ENZ-51002) appear to target the inner mitochondrial membrane in mammalian cells, which…
Tue, Jul 18, 2023
Would COX activity assay kit, ADI-907-003 work with human plasma and mouse cell lysate samples?
COX activity assay kit, ADI-907-003 https://www.enzolifesciences.com/fileadmin/files/manual/ADI-907-003_insert.pdf is designed for the quantitative determination of COX activity in biological samples. However, we…
Tue, Jul 18, 2023
Can some guidance be provided to detect the activity of COX1 and COX2 from human plasma, and advise assay design with the Ibuprofen, meloxicam, known COX-2 inhibitors for COX activity assay kit, ADI-907-003?
We have not specifically validated this kit with human plasma samples. Page 5 of user manual provides the work flow. This provides…
Tue, Jul 18, 2023
What starting volume is required for COX activity assay kit, ADI-907-003?
For COX activity assay kit, ADI-907-003 we recommend starting volumes of 50 µL COX-I or COX-II preparations. Actual sample volumes required…
Tue, Jul 18, 2023