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ELISA Immunoassays
Is the kit specific to Endothelin-1?
The antibodies used in this ELISA are highly specific with 100% cross-reactivity to ET-1, 21% to ET-2, 3.6% to ET-3…
Tue, Jul 18, 2023
The sensitivity of ADI-900-020A is listed as 0.41 pg/mL, whereas the standard range only goes as low as 0.78 pg/mL. What is the limit of detection of the assay?
Limit of detection is a statistical measurement meant to indicate the lowest concentration that the assay can differentiate from zero…
Tue, Jul 18, 2023
I plan to use your ADI-900-020A kit to measure Endothelin-1 (ET-1) concentration in cell culture supernatant. Samples contain 10% fetal bovine serum (FBS). Will I need to extract them?
The ET-1 ELISA kit is suitable for use with tissue culture supernatants containing up to 10% FBS. In our spike…
Tue, Jul 18, 2023
I bought your ADI-900-020A kit and I just realized that the manual indicates to avoid lipemic samples. My plasma samples are lipemic. Is there a way I can try measuring ET-1 concentration with your kit?
How lipid can potentially interfere in the assay has not specifically been tested, but you might want to consider delipidating your…
Tue, Jul 18, 2023
How essential is the addition of aprotinin when collecting blood samples?
The aprotinin is included to help prevent any degradation of ET-1, but also to prevent any further cleavage of the…
Tue, Jul 18, 2023
Which C18 reverse phase columns can be used for sample extraction with the LTB4 ELISA kit?
The Honeywell Burdick & Jackson® Non-Polar Silica Based SPE Columns, Cat. No. 9002, is the recommended C18 reverse phase column for…
Tue, Jul 18, 2023
When do I need to extract my samples for use with the LTB4 ELISA kit?
Sample extraction for this assay may only be needed if you have low LTB4 levels, so your samples cannot be…
Tue, Jul 18, 2023
Which species, other than human, mouse or rat, can these HSP70 ELISA kits detect?
ADI-EKS-700B, ADI-EKS-715, and ENZ-KIT-101 have not been tested with other species; so, it cannot be said with certainty whether it would be…
Tue, Jul 18, 2023
Which species can be analyzed with the LTB4 ELISA kit?
Leukotriene B4 (LTB4) is a small molecule and thus a defined chemical entity that does not change in between species…
Tue, Jul 18, 2023
Will the LTB4 ELISA kit work with serum samples?
This kit has not been validated internally for serum. Sample types that have been validated internally are ETDA plasma, cultured media, saliva…
Tue, Jul 18, 2023