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ELISA Immunoassays
How should serum samples be prepared for use with the NGAL (human) ELISA kit?
Collect whole blood in a covered test tube. If commercially available tubes are to be used, the researcher should use…
Tue, Jul 18, 2023
How should plasma samples be prepared for use with the NGAL (human) ELISA kit?
Collect whole blood into commercially available anticoagulant-treated tubes e.g., EDTA-treated (lavender tops) or citrate-treated (light blue tops). Heparinized tubes (green…
Tue, Jul 18, 2023
How long can I use an ELISA kit after expiry?
Once ELISA kits are expired their performance can no longer be guaranteed. However, ELISA kits do not typically stop working entirely on their…
Tue, Jul 18, 2023
I have always seen the TMB Substrate Solution as a clear solution, but today the solution had significant green-blue tinge. What might have caused this color change?
The color tinge in the TMB substrate is due to contamination by the conjugate. To avoid contamination it is important…
Tue, Jul 18, 2023
Can I use the Testosterone ELISA kit with human plasma and serum samples?
Sample types that have been validated are indicated on our website in the field “Application” and in the kit insert.…
Tue, Jul 18, 2023
Can the Testosterone ELISA kit be used with samples from any species?
Testosterone is conserved across all species and the antibody provided in the kit will bind to Testosterone independently from the…
Tue, Jul 18, 2023
How should I treat samples with the steroid displacement reagent (SDR) for analysis with the Testosterone ELISA kit?
The Steroid Displacement Reagent should be used to treat appropriate samples in the following manner: Add 1µl of SDR per…
Tue, Jul 18, 2023
Can the Progesterone ELISA kit be used with samples from any species?
Progesterone is conserved across all species and the antibody provided in the kit will bind to progesterone independently from the…
Tue, Jul 18, 2023
How should I treat samples with the steroid displacement reagent (SDR) for analysis with the Progesterone ELISA kit?
The Steroid Displacement Reagent should be used to treat appropriate samples in the following manner: Add 1µl of SDR per…
Tue, Jul 18, 2023
Can the Cortisol ELISA kit be used with samples from any species?
Cortisol is conserved across all species and the antibody provided in the kit will bind to cortisol independently from the…
Tue, Jul 18, 2023