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ELISA Immunoassays
I have different cell lysates which I would like to run with ADI-EKS-895, HSP90a (human) ELISA kit, but I am not sure if the formulation of my lysis buffer is identical to the extraction buffer present in the kit. Will I be able to analyze my samples
The exact formulation of our extraction buffer is proprietary and cannot be disclosed. The buffer is optimized to achieve complete…
Tue, Jul 18, 2023
Are the polyclonal antibodies against E.coli, CHO, and HEK293T host cell proteins purified and by what method?
These antibodies were indeed purified using MabSelect™ (Protein A affinity column) from GE Healthcare.
Tue, Jul 18, 2023
Why do you use protein A affinity columns to purify the antibodies included in ENZ-KIT127, ENZ-KIT128, and ENZ-KIT162 instead of HCP affinity columns?
The main reason is product consistency. Protein A affinity columns have been around for quite a while. They are well…
Tue, Jul 18, 2023
How were the CHO host cell protein standards generated?
The CHO host cell protein standards were prepared from multiple CHO K1 cell lines growing in suspension and in chemically…
Tue, Jul 18, 2023
Can the ADI-EKS-895 be used to measure mouse HSP90a?
No, the capture antibody does not react with mouse Hsp90a, which prevents the assay from being able to recognize those…
Tue, Jul 18, 2023
Can I use the kit ADI-EKS-895 to measure HSP90a activity?
ADI-EKS-895 is used to measure the total amount of human HSP90a in a given sample. It cannot be used for…
Tue, Jul 18, 2023
How should I store reconstituted standard, antibody and conjugatefor ADI-900-175?
The standard, antibody and conjugate are single use items and should to be stored at -20°C. Any leftover of reconstituted…
Tue, Jul 18, 2023
I bought your ADI-900-020A kit and I just realized that the manual indicates to avoid lipemic samples. My plasma samples are lipemic. Is there a way I can try measuring ET-1 concentration with your kit?
How lipid can potentially interfere in the assay has not specifically been tested, but you might want to consider delipidating your…
Tue, Jul 18, 2023
Can the Serotonin ELISA kit, ADI-900-175, be used with samples from any species?
Serotonin is conserved across all species and the antibody provided in the kit will bind to Serotonin independently from the…
Tue, Jul 18, 2023
Which type of plasma samples can be used with your ADI-900-020A, Endothelin-1 ELISA kit (Heparin, EDTA or Citrate)?
The kit has been validated using EDTA plasma samples. Heparin and citrate plasma were not tested. We generally recommend EDTA plasma for…
Tue, Jul 18, 2023