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ELISA Immunoassays
Can Standards and Samples be derivatized on different days with Protein Carbonyl ELISA kit, ALX-850-312?
Standards, Controls and samples should be treated simultaneously with DNP on the same day in parallel to maintain consistency. When…
Tue, Jul 18, 2023
Can Excel be used to plot read outs from ELISA? How to calculate reads from ELISA using Excel?
Our recommendation is to analyze ELISA reads by 4P curve fit. Many plate readers do have the ability to perform…
Tue, Jul 18, 2023
Why is 4P curve fit curve recommended for ELISA data analysis and where can I find access to this data analysis software?
For the most accurate results, we normally recommend to use a 4-parameter logistic (4-PL) curve fitting software, as 4-PL fitting…
Tue, Jul 18, 2023
Does 17ß-Estradiol ELISA kit, ADI-900-008 cross react with Estradiol 3-benzoate?
Estradiol 3-benzoate has not been determined for cross reactivity with17ß-Estradiol ELISA kit (ADI-900-008). The cross relativities for a number of…
Tue, Jul 18, 2023
How is the cross reactivity for 17ß-Estradiol related steroid compounds determined with 17ß-Estradiol ELISA kit, ADI-900-008?
During the develomment of 17ß-Estradiol ELISA kit (ADI-900-008), the cross reactivities for a number of related steroid compounds was determined…
Tue, Jul 18, 2023
Is solvent extraction of samples required TXB2 ELISA kit (ADI-900-002 and ADI-901-002)?
With many ELISA kits, including TXB2 ELISA kit (ADI-900-002 and ADI-901-002), extraction is required if the levels of marker is…
Tue, Jul 18, 2023
27 ml Assay Buffer is provided in ADI-901-002, ADI-901-153A, ADI-901-173 and in several other 5 plate ELSIA kits. Would this amount of buffer be sufficient for all of the samples after preparing standards?
Assay buffer in 5x 96 plate kits (e.g. in ADI-901-002, ADI-901-153A, ADI-901-173) is provided as 10x Concentrate and this requires…
Tue, Jul 18, 2023
What is the recommended dilutions for plasma samples obtained from subjects treated with aspirin for TXB2 ELISA kit (ADI-900-002 and ADI-901-002)?
When the TBX2 ELISA kit (ADI-900-002 and ADI-901-002) was developed with pooled samples, 1:100 dilution worked. So, this is a…
Tue, Jul 18, 2023
Does Enzo Protein A ELISA kit, ADI-900-057, detect Protein A purified on MabSelect PrismA and MabSelect SuRe™ resins?
Protein A ELISA kit (ADI-900-057) detects ligand that bind to GE bound MabSelect SuRe™ resin. We have not determined the…
Tue, Jul 18, 2023
Is charcoal-stripped FBS and serum free DMEM required for spike and recovery experiment with cell culture sample with Progesterone ELISA kit, ADI-900-011?
ANS: We suggest to prepare standard curve in the same unconditioned media used to culture samples. General recommendation for diluting…
Tue, Jul 18, 2023