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ELISA Immunoassays
Is charcoal-stripped FBS and serum free DMEM required for spike and recovery experiment with cell culture sample with Progesterone ELISA kit, ADI-900-011?
ANS: We suggest to prepare standard curve in the same unconditioned media used to culture samples. General recommendation for diluting…
Tue, Jul 18, 2023
Can AMP’D® ELISA Signal Amplification Kit ENZ-KIT-100 be used with any commercially available ELISA kit to amplify signals with alkaline phosphatase (AP) substrates?
ANS: AMP'D® ELISA Signal Amplification kit, ENZ-KIT-100 has a signal amplification substrate containing NADPH and enzyme system for increasing the sensitivity…
Tue, Jul 18, 2023
How do I choose the correct dilution factor for my samples to be used in my ELISA ?
Optimizing sample preparation and dilution is a key step in the validation of any ELISA assay. It is crucial that…
Tue, Jul 18, 2023
For the Protein A ELISA kit, ADI-900-057, would the readings be affected if the wells were to dry out when residual wash buffer is blotted out?
In general with any ELISA, including the Protein A ELISA kit (ADI-900-057), care should be taken not to allow the…
Tue, Jul 18, 2023
Does the Protein A ELISA kit recognize denatured and non-denatured protein A?
A variety of Protein A constructs have been tested in our Protein A ELISA kit. Please refer to the product…
Tue, Jul 18, 2023
For the Protein A ELISA kit (ADI-900-057) would residual wash buffer affect the assay?
In general with any ELISA, including the Protein A ELISA kit (ADI-900-057), residual wash buffer can interfere with the assay…
Tue, Jul 18, 2023
Why do you use protein A affinity columns to purify the antibodies included in ENZ-KIT127, ENZ-KIT128, and ENZ-KIT162 instead of HCP affinity columns?
The main reason is product consistency. Protein A affinity columns have been around for quite a while. They are well…
Tue, Jul 18, 2023
I have different cell lysates which I would like to run with ADI-EKS-895, HSP90a (human) ELISA kit, but I am not sure if the formulation of my lysis buffer is identical to the extraction buffer present in the kit. Will I be able to analyze my samples
The exact formulation of our extraction buffer is proprietary and cannot be disclosed. The buffer is optimized to achieve complete…
Tue, Jul 18, 2023
Can the ADI-EKS-895 be used to measure mouse HSP90a?
No, the capture antibody does not react with mouse Hsp90a, which prevents the assay from being able to recognize those…
Tue, Jul 18, 2023
Which type of plasma sample do you recommend for using with ADI-900-175?
The kit was validated with citrate plasma samples. EDTA plasma will likely work fine as long as the final EDTA…
Tue, Jul 18, 2023