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ELISA Immunoassays
What is the suggestion for using part of the ADI-900-057, Protein A ELISA kit then re-using the remainder of the kit at a later date?
In general, we would not advise holding the entire kit at 5-6 hours at ambient temperature if this can be…
Tue, Jul 18, 2023
What does Standard Diluent refer to in several ELISA user manual when cell culture supernatant is used?
Standard diluent refers to either the tissue culture media (a.k.a. non-conditioned media) or the assay buffer provided. So, the supernatant…
Tue, Jul 18, 2023
Can Sample diluent (80-1504) be used to dilute antibody if one runs out of antibody diluent (80-1759) with HO-1 (human), ELISA kit, ADI-EKS-800?
We do not recommend using Sample diluent (80-1504) to dilute antibody instead of antibody diluent (80-1759) with HO-1 (human), ELISA…
Tue, Jul 18, 2023
What information does Lot specific Certificate of Analysis for Protein A ELIA kit, ADI-900-057 have?
Lot specific Certificate of Analysis for Protein A ELISA kit (ADI-900-057) lists: assay range, OD values for High, Medium, Low,…
Tue, Jul 18, 2023
Where do I find validation data for Protein A ELISA kit, ADI-900-057 since this is not listed on datasheet?
Description of Validation experiments and the data for Protein A ELISA kit (ADI-900-057) are listed in pages from 9-11 of…
Tue, Jul 18, 2023
How is the Protein A ELISA kit, ADI-900-057 validated?
The Protein A ELISA Kit (ADI-900-057) recognizes natural and recombinant forms of Protein A. Four Protein A constructs were evaluated…
Tue, Jul 18, 2023
Is there a requirement for bridging when changing lots of E. coli host cell protein ELISA kit, ENZ-KIT127?
A bridging study is required if this is a part of defined or specified SOP for a facility or a…
Tue, Jul 18, 2023
How are Quality control tests for each lot of E.coli host cell protein ELISA kit, ENZ-KIT127 performed?
Quality control testing is performed by randomly selecting multiple plates from each lot. All lot-specific components are tested together using…
Tue, Jul 18, 2023
How long can anti-DNP biotin antibody and streptavidin-HRP be stored once reconstituted for use with Protein Carbonyl ELISA, ALX-850-312?
Anti-DNP-biotin antibody (G) can be stored at -20 °C, for 1-2 weeks and streptavidin-horseradish peroxidase (H) can also be stored…
Tue, Jul 18, 2023
Can samples, controls and standards be stored once they are DNP derivatized Protein Carbonyl ELISA kit, ALX-850-312?
Storing samples, controls or standards after derivatization is not recommended. So, careful planning of work flow is needed to complete…
Tue, Jul 18, 2023