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Knowledge Base
Can the microplate be read following addition of ROS/Superoxide Detection Solution with ROS-ID® Total ROS/Superoxide detection kit, ENZ-51010?
Yes, plates can be read using bottom reading option without removing the ROS/Superoxide Detection Solution and without washing.
Tue, Jul 18, 2023
How do we perform QC for E. coli host cell protein ELISA kit, ENZ-KIT127?
Quality control for E. coli host cell protein ELISA kit (ENZ-KIT127) is performed by randomly selecting multiple plates from each…
Tue, Jul 18, 2023
Buffer salts in 10x PBS-T wash buffer in E. coli host cell protein ELISA kit, ENZ-KIT127 has precipitated upon storage at 4°C. Is this usual and how to dissolve the salts?
On occasions, buffer salts precipitate out of solution at 4°C. Warming the bottle containing buffer at 37°C and frequent vortexing would…
Tue, Jul 18, 2023
How does PATHO-GENE® HPV Type 11 Probe, ENZ-GEN151 work?
The ENZO PATHO-GENE® HPV Type 11 Probe (ENZ-GEN151) is a biotin-labeled DNA probe designed to hybridize to HPV Type 11 DNA sequences. The…
Tue, Jul 18, 2023
Can cells be fixed with 4% paraformaldehyde with the CYTO-ID® Autophagy detection kits (ENZ-51031 and ENZ-KIT175)?
A fixation step may be included when using the CYTO-ID® Autophagy detection kits (ENZ-51031 and ENZ-KIT175), but it needs to…
Tue, Jul 18, 2023
What is the recommended solvent to dissolve Z-Gly-Gly-Leu-AMC, BML-ZW8505?
Z-Gly-Gly-Leu-AMC, BML-ZW8505-0005 can be dissolved in methanol. Maximum solubility is 10 mg/ml to obtain a clear colorless solution
Tue, Jul 18, 2023
Is Autophagy Detection reagent in ENZ-51031 and ENZ-KIT175 compatible with anti-fluorescence quenching agent and fluoromount-G?
The CYTO-ID® Autophagy Detection reagent in ENZ-51031 and ENZ-KIT175 kits is compatible with anti-fluorescence quenching agent and Fluoromount-G.
Tue, Jul 18, 2023
Is there a requirement for cell lysis with LEADING LIGHT® Wnt Reporter Assay, ENZ-61001
LEADING LIGHT® Wnt Reporter Assay (ENZ-61001) is a cell-based luciferase and does not require lysis and chemiluminescence signals can be…
Tue, Jul 18, 2023
Do we offer a protocol for Z-Gly-Gly-Leu-AMC, BML-ZW8505?
This peptide should be re-suspended in methanol at a concentration of 10mg/ml to give a clear colorless solution. The following…
Tue, Jul 18, 2023
Can CHO host cell protein standards (ENZ-PRT122) be used as internal control in Western Blot or as standards in CHO Host cell protein ELISA kit (ENZ-KIT128)?
CHO host cell protein standards (ENZ-PRT122) can be used in Western blot or in an ELISA. With CHO Host cell…
Tue, Jul 18, 2023