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When using an IMMUNOSET® ELISA development set, will my plate coating technique affect the precision of my plates and the overall assay?
It is possible for plate coating technique to lead to poor precision. Please refer to the insert for specific recommendations.
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, how can I get the greatest stability out of the coated plates?
After blocking, shake out all excess solution and store desiccated at 4°C.
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, how can I avoid evaporation of the solutions while coating, blocking, and assaying?
Seal the plates with a plate sealer, plastic wrap, or foil during incubations.
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, can I use other Streptavidin-HRP solutions?
Yes, however the reagent should be optimized (titered) to assure acceptable signal intensity and background are achieved.
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, is it really necessary to add stop solution before reading the plate?
While most plate readers have the ability to read optical density in the blue spectrum, they are usually more sensitive…
Tue, Jul 18, 2023
How do I know whether to use the Nitric Oxide (NO<sub>2-</sub>/NO<sub>3-</sub>) detection kit or the Nitric Oxide (total) detection kit?
The regular kit measures both major metabolic products of NO — nitrate and nitrite. The Total kit measures these metabolites…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, what kind of substrate should I use to develop the plate?
Any colorimetric peroxidase substrate compatible with the enzyme of choice as well as your plate reader should be acceptable. See…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, can I alter the incubation times or temperatures? Do I need to shake my plate?
The assay procedure has been optimized, and any alterations will require validation.
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, can I alter the volume or concentration of antibody for coating?
Altering the volume or concentration of the coating antibody will have an impact on assay performance. Users should validate the…
Tue, Jul 18, 2023