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I am seeing much higher than expected nitrate levels. What could be the problem?
Many times the water source in the lab is the culprit. The water is only as pure as the last…
Tue, Jul 18, 2023
It says in the insert that samples containing high levels of NADPH need to be diluted sufficiently. How can I determine the NADPH levels and dilution prior to running the Nitric Oxide detection assay?
We have found that 0.5-1mM NADPH will inhibit the Griess color reaction slightly. If this amount of NADPH is present…
Tue, Jul 18, 2023
What type of 10,000 MWCO filter is best to use prior to using the Nitric Oxide detection kits?
The user has a choice between a spin-filter, a syringe-type filter, or a 96-well filtration plate format. Generally, a filter…
Tue, Jul 18, 2023
Why do I have to filter my samples through a 10,000 MWCO filter prior to using the Nitric Oxide detection kits?
In order to get the best sensitivity, samples need to be filtered to remove or reduce interfering substances. Using a…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, can I use other Streptavidin-HRP solutions?
Yes, however the reagent should be optimized (titered) to assure acceptable signal intensity and background are achieved.
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, is it really necessary to add stop solution before reading the plate?
While most plate readers have the ability to read optical density in the blue spectrum, they are usually more sensitive…
Tue, Jul 18, 2023
How do I know whether to use the Nitric Oxide (NO<sub>2-</sub>/NO<sub>3-</sub>) detection kit or the Nitric Oxide (total) detection kit?
The regular kit measures both major metabolic products of NO — nitrate and nitrite. The Total kit measures these metabolites…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, what kind of substrate should I use to develop the plate?
Any colorimetric peroxidase substrate compatible with the enzyme of choice as well as your plate reader should be acceptable. See…
Tue, Jul 18, 2023
When using an IMMUNOSET® ELISA development set, can I alter the incubation times or temperatures? Do I need to shake my plate?
The assay procedure has been optimized, and any alterations will require validation.
Tue, Jul 18, 2023
Can I measure tissue NO with the Nitric Oxide detection kits?
Yes. Samples can be homogenized in the provided Reaction Buffer and then centrifuged. The supernatant should then be filtered through…
Tue, Jul 18, 2023