Can I store acetylated samples for cAMP/cGMP quantification at a later time?
No. We recommend that only enough samples needed for the current run be acetylated. Samples are more stable when stored…
Tue, Jul 18, 2023
What if my cells are not sufficiently lyzed for cAMP/cGMP quantification after the addition of 0.1M HCl?
Cell or tissue lysis can be enhanced by adding 0.1-1% Triton X-100 to 0.1M HCl prior to use. When used…
Tue, Jul 18, 2023
Which protein assay is compatible with lysates obtained with HCl treatment?
This question is actually better suited for a company supplying the protein assay you wish to use. Thermo, for example,…
Tue, Jul 18, 2023
Can I use my own lysis buffer for the preparation of my cell lysates prior to cAMP/cGMP quantification?
The kit has been optimized for using HCl to both lyse and dilute your standards and samples while used in…
Tue, Jul 18, 2023
Can samples diluted with the Assay Buffer from the cAMP ELISA kit be used with the cGMP ELISA kit and vice versa?
The Assay Buffers in these two kits have the same catalogue number and are exactly identical. As long as the…
Tue, Jul 18, 2023
Can samples diluted with the Assay Buffer from the cAMP Direct ELISA kit be used with the cGMP Direct ELISA kit and vice versa?
The Assay Buffers in these two kits have the same catalogue number and are exactly identical. As long as the end…
Tue, Jul 18, 2023
Can samples diluted with the Assay Buffer from the cAMP complete ELISA kit be used with the cGMP complete ELISA kit and vice versa?
The Assay Buffers in these two kits have the same catalogue number and are exactly identical. As long as the…
Tue, Jul 18, 2023
Can I detect cytoplasmic cAMP/cGMP with these ELISA kits?
No, these are colorimetric ELISA kits for the quantification of extra-cellular cAMP/cGMP in culture supernatants, serum, and saliva from any…
Tue, Jul 18, 2023
Can I detect cytoplasmic cAMP/cGMP with these Direct ELISA kits?
These are colorimetric ELISA kits developed specifically for the measurement of intracellular cAMP/cGMP in cell or tissue lysates from any…
Tue, Jul 18, 2023
Can I detect cytoplasmic cAMP/cGMP with these Complete ELISA kits?
These are colorimetric ELISA kits for the quantification of both intra- and extra-cellular cAMP/cGMP in cell lysates, culture supernatants, serum,…
Tue, Jul 18, 2023
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