Lab Essentials
AMPIVIEW® RNA probes
Enabling Your Projects 
GMP Services
Bulk Solutions
Research Travel Grant
Have You Published Using an Enzo Product?
Knowledge Base
Values for this assay are reported as pmol/well. How can I convert the values obtained by this kit into a value that is more commonly reported in the literature, such as pmol/L (pM)?
Since the volume of sample added to each well is 50µL, results can be converted to molarity by multiplying by…
Tue, Jul 18, 2023
Will the cytokine ELISA kits from one species work with another species?
This will depend on the species homology. Human cytokine ELISA kits will generally not measure cytokines in other species. Mouse…
Tue, Jul 18, 2023
What is the purpose of treating samples with 5% metaphosphoric acid (MPA)?
MPA should be added to fresh samples prior to sample handling or sample storage. That is required to deproteinize samples…
Tue, Jul 18, 2023
Why and how should I treat my samples and the standard with 4-Vinylpyridine (4-VP)?
In order to measure oxidized glutathione (GSSG), you must eliminate GSH in the cell extract by treating with 4-VP, a…
Tue, Jul 18, 2023
After having measured the total glutathione content, is it possible to use the same samples on the plate so as to continue the procedure and measure the oxidized form of glutathione?
The measurements of total and oxidized glutathione are two completely different events; it is therefore not possible to use the…
Tue, Jul 18, 2023
My first assay for total glutathione worked well, but when I repeated the assay to test the oxidized samples, the curve was essentially flat-to-negative.
Oxidized glutathione is very difficult to detect because it is rapidly turned over and converted, so there is only a…
Tue, Jul 18, 2023
What can interfere with the assay?
Reducing agents (such as ascorbic acid, beta-mercaptoethanol, dithiothreitol, and cysteine) and thiol reactive compounds (such as maleimides) are known to…
Tue, Jul 18, 2023
Can whole blood samples be used?
High background may result from the presence of hemoglobin in your sample. Hemoglobin (Hb) has a significant absorption at 340…
Tue, Jul 18, 2023
Glossary
Antibody: An immunoglobulin that recognizes some portion of an antigen molecule. The antibody contains a species-specific Fc region and antigen-specific…
Tue, Jul 18, 2023
What is the difference between the 8-iso-PGF<sub><span style="font-size: 10pt;">2a</span></sub> ELISA kit and the Direct 8-iso-PGF<sub><span style="font-size: 10pt;">2a</span></sub> ELISA kit?
The regular 8-iso-PGF2a kit is designed to measure free isoprostane in samples. Direct 8-iso-PGF2a measures total isoprostane levels, both free…
Tue, Jul 18, 2023