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Will the LTB4 ELISA kit work with serum samples?
This kit has not been validated internally for serum. Sample types that have been validated internally are ETDA plasma, cultured media, saliva…
Tue, Jul 18, 2023
Which ELISA kit should I use to measure the levels of HSP70 in my serum/plasma samples?
The HSP70 ELISA kit (ADI-EKS-700B) was designed to measure HSP70 in cell and tissue lysates, and is not typically sensitive…
Tue, Jul 18, 2023
Which antibodies are used in your HSP70 ELISA kits (ADI-EKS-700B, ADI-EKS-715, and ENZ-KIT-101)?
This information is considered to be proprietary and cannot be disclosed. The antibody recognizes an internal epitope of HSP70. The…
Tue, Jul 18, 2023
Can I use your HIGHDEF® Chromogens with an alternative detection system (e.g. ABC)?
In theory, it is possible to use the HIGHDEF® Chromogens with an alternative detection system. It is recommended, however, to use our POLYVIEW® PLUS…
Tue, Jul 18, 2023
According to the instruction manual, HIGHDEF® red IHC chromogen (AP, plus) (ADI-950-141) produces a red color, ranging from pink to brilliant dark red. What does that depend on?
The color intensity depends on the levels of expression of the antigen of interest.
Tue, Jul 18, 2023
When doing a four-color IHC staining, is there a risk that the colors will fade during the process?
It is recommended to optimize with each step in order to avoid fading of each color due to time-lag between…
Tue, Jul 18, 2023
Is the ISH/IHC Peroxidase block solution provided in the MULTIVIEW® PLUS IHC kit (ENZ-KIT181) the same as the IHC tissue primer (ADI-950-234)?
The peroxidase blocker solution is functionally the same as the IHC tissue primer but different in its formulation. The IHC tissue…
Tue, Jul 18, 2023
I plan to perform multiple IHC staining using your MULTIVIEW® PLUS IHC kit. What are your recommendations in case the targets co-localize?
In IHC, designing the experiment correctly is very critical. In the ideal situation, you will label unique proteins or antigens…
Tue, Jul 18, 2023
Will the HO-1 (h) ELISA cross react with HO-2 and HO-3?
This assay is specific for human HO-1 and will not cross react with human HO-2 and HO-3, the other known…
Tue, Jul 18, 2023
How can cross-reaction of detection reagents with primary antibodies be avoided using the MULTIVIEW® PLUS kit (ENZ-KIT181)?
Regarding the MULTIVIEW® PLUS (mouse-HRP/mouse-AP) IHC kit (Brown/Green), sufficient washes between antibody, detection, and chromogen steps (3 x 1 minute…
Tue, Jul 18, 2023