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Knowledge Base
Which species, other than human, mouse or rat, can these HSP70 ELISA kits detect?
ADI-EKS-700B, ADI-EKS-715, and ENZ-KIT-101 have not been tested with other species; so, it cannot be said with certainty whether it would be…
Tue, Jul 18, 2023
How much sample do I need to use and what are the suggested dilutions when using ADI-EKS-700B, ADI-EKS-715, ADI-EKS-725A, ADI-EKS-750, and ENZ-KIT-101?
The amount of sample required and the dilutions needed for your assay depend upon the amount of HSP70, HSP70B, or…
Tue, Jul 18, 2023
Can I detect constitutive HSP70 with ADI-EKS-700B or ADI-EKS-715?
These assays are specific for induced HSP70 and will not detect constitutive HSP70. Taking this into account, you need to…
Tue, Jul 18, 2023
How to prevent the formation of bubbles when using the HIGHDEF® IHC mount (ADI-950-261)?
In order to prevent bubble formation, please follow these recommendations:1) Remove red tip from the bottle and cut the tip off…
Tue, Jul 18, 2023
What is the difference between the mounting media ADI-950-260 and ADI-950-261?
HIGHDEF® IHC fluoromount (ADI-950-260) was specifically designed as an antifade reagent to cover tissues stained with fluorescent dyes. HIGHDEF® IHC…
Tue, Jul 18, 2023
Can I use your HIGHDEF® Chromogens with an alternative detection system (e.g. ABC)?
In theory, it is possible to use the HIGHDEF® Chromogens with an alternative detection system. It is recommended, however, to use our POLYVIEW® PLUS…
Tue, Jul 18, 2023
According to the instruction manual, HIGHDEF® red IHC chromogen (AP, plus) (ADI-950-141) produces a red color, ranging from pink to brilliant dark red. What does that depend on?
The color intensity depends on the levels of expression of the antigen of interest.
Tue, Jul 18, 2023
What is the difference between ADI-950-140 and ADI-950-141?
Some red alkaline phosphatase chromogens are unstable upon mixing. This can be problematic where an automated slide strainer is used. The…
Tue, Jul 18, 2023
Why is the SAVIEW® PLUS detection technology superior compared to the traditional ABC detection method?
The high affinity of the non-covalent interaction between biotin and streptavidin (Kd 4×10-14 M) forms the basis for our SAVIEW®…
Tue, Jul 18, 2023
When doing a four-color IHC staining, is there a risk that the colors will fade during the process?
It is recommended to optimize with each step in order to avoid fading of each color due to time-lag between…
Tue, Jul 18, 2023