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Which species, other than human, mouse or rat, can these HSP70 ELISA kits detect?
ADI-EKS-700B, ADI-EKS-715, and ENZ-KIT-101 have not been tested with other species; so, it cannot be said with certainty whether it would be…
Tue, Jul 18, 2023
How much sample do I need to use and what are the suggested dilutions when using ADI-EKS-700B, ADI-EKS-715, ADI-EKS-725A, ADI-EKS-750, and ENZ-KIT-101?
The amount of sample required and the dilutions needed for your assay depend upon the amount of HSP70, HSP70B, or…
Tue, Jul 18, 2023
Can I detect constitutive HSP70 with ADI-EKS-700B or ADI-EKS-715?
These assays are specific for induced HSP70 and will not detect constitutive HSP70. Taking this into account, you need to…
Tue, Jul 18, 2023
Can I use your HIGHDEF® Chromogens with an alternative detection system (e.g. ABC)?
In theory, it is possible to use the HIGHDEF® Chromogens with an alternative detection system. It is recommended, however, to use our POLYVIEW® PLUS…
Tue, Jul 18, 2023
According to the instruction manual, HIGHDEF® red IHC chromogen (AP, plus) (ADI-950-141) produces a red color, ranging from pink to brilliant dark red. What does that depend on?
The color intensity depends on the levels of expression of the antigen of interest.
Tue, Jul 18, 2023
When doing a four-color IHC staining, is there a risk that the colors will fade during the process?
It is recommended to optimize with each step in order to avoid fading of each color due to time-lag between…
Tue, Jul 18, 2023
Is the ISH/IHC Peroxidase block solution provided in the MULTIVIEW® PLUS IHC kit (ENZ-KIT181) the same as the IHC tissue primer (ADI-950-234)?
The peroxidase blocker solution is functionally the same as the IHC tissue primer but different in its formulation. The IHC tissue…
Tue, Jul 18, 2023
I plan to perform multiple IHC staining using your MULTIVIEW® PLUS IHC kit. What are your recommendations in case the targets co-localize?
In IHC, designing the experiment correctly is very critical. In the ideal situation, you will label unique proteins or antigens…
Tue, Jul 18, 2023
What is the nature of the recombinant HO-1 standard provided in the kit ADI-EKS-800, HO-1 (human), ELISA kit?
The standard is a recombinant human HO-1 (UniProt ID: P09601) expressed in E.coli.
Tue, Jul 18, 2023
Will the HO-1 (h) ELISA cross react with HO-2 and HO-3?
This assay is specific for human HO-1 and will not cross react with human HO-2 and HO-3, the other known…
Tue, Jul 18, 2023