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Knowledge Base
I am planning to quantify 8-OHdG in cancer patient samples using ADI-EKS-350. This kit can be used to test urine, serum, and saliva samples. Which sample should I use?
Urine is usually the sample of choice for 8-OHdG when looking at DNA damage; however, which sample may be best…
Tue, Jul 18, 2023
What do I need to do if my antibody concentration is higher than the 50 µg/mL indicated in the manual for ADI-900-057?
If you have samples with an antibody concentration higher than 50 µg/mL, you just need to dilute these samples down to…
Tue, Jul 18, 2023
Can I measure the levels of 8-OHdG in cell or tissue extracts?
The order of events happening in the cell is that the damaged DNA is modified, halting further transcription. Repair of…
Tue, Jul 18, 2023
What is a good positive control to study protein aggregation in cells?
MG132 treated cells would serve as positive control to determine background noise compared to signal along with untreated negative control.
Tue, Jul 18, 2023
Can the Proteostat kit be used to study protein aggregation with gram negative bacteria?
LPS in outer membrane of gram negative bacteria can be problematic. The permeabilizing buffer described in the manual, would remove…
Tue, Jul 18, 2023
What is the suggested remedy to minimize signal in the FITC emission filter while co-staining other proteins?
The excitation and emission spectra of the dye is provided on page 12 of the kit manual. The spectrum indicates…
Tue, Jul 18, 2023
Can the FluoForte® Calcium assay kit be used to monitor Ca2+ flux in nicotinic acetylcholine receptors on Flexstation. Can the FluoForte dye monitor Ca2+ in our model system given that FluoForte dye has been validated only in GPCR signaling path
While no specific references are available for application of FluoForte to assay nicotinic receptor, there are numerous publications regarding the…
Tue, Jul 18, 2023
How much sample do I need to use and what are the suggested dilutions when using ADI-EKS-700B, ADI-EKS-715, ADI-EKS-725A, ADI-EKS-750, and ENZ-KIT-101?
The amount of sample required and the dilutions needed for your assay depend upon the amount of HSP70, HSP70B, or…
Tue, Jul 18, 2023
Can I detect constitutive HSP70 with ADI-EKS-700B or ADI-EKS-715?
These assays are specific for induced HSP70 and will not detect constitutive HSP70. Taking this into account, you need to…
Tue, Jul 18, 2023
Does your Protein A ELISA kit (ADI-900-057) detect both “natural” and “unnatural” Protein A?
As is illustrated in the figure included in the data sheet of ADI-900-057, this ELISA kit can detect both native…
Tue, Jul 18, 2023