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How can cross-reaction of detection reagents with primary antibodies be avoided using the MULTIVIEW® PLUS kit (ENZ-KIT181)?
Regarding the MULTIVIEW® PLUS (mouse-HRP/mouse-AP) IHC kit (Brown/Green), sufficient washes between antibody, detection, and chromogen steps (3 x 1 minute…
Tue, Jul 18, 2023
What are your recommendations as for mounting media and post-processing for your HIGHDEF® chromogens?
All our chromogens should be used preferentially with an aqueous-based mounting medium (e.g. ADI-950-261) for a better resolution and a…
Tue, Jul 18, 2023
How should I handle and store cell lysates if I cannot assay them immediately?
In order to prevent HO-1 degradation it is important to add protease inhibitors in the lysis buffer just prior to…
Tue, Jul 18, 2023
Why is the SAVIEW® PLUS detection technology superior compared to the traditional ABC detection method?
The high affinity of the non-covalent interaction between biotin and streptavidin (Kd 4×10-14 M) forms the basis for our SAVIEW®…
Tue, Jul 18, 2023
What is the difference between ADI-950-140 and ADI-950-141?
Some red alkaline phosphatase chromogens are unstable upon mixing. This can be problematic where an automated slide strainer is used. The…
Tue, Jul 18, 2023
What is the difference between the mounting media ADI-950-260 and ADI-950-261?
HIGHDEF® IHC fluoromount (ADI-950-260) was specifically designed as an antifade reagent to cover tissues stained with fluorescent dyes. HIGHDEF® IHC…
Tue, Jul 18, 2023
How to prevent the formation of bubbles when using the HIGHDEF® IHC mount (ADI-950-261)?
In order to prevent bubble formation, please follow these recommendations:1) Remove red tip from the bottle and cut the tip off…
Tue, Jul 18, 2023
Does the product ADI-EKS-800, HO-1 (human), ELISA kit also work for human plasma and serum?
The kit has been validated for the measurement of intracellular HO-1 in cell lysates and tissue extracts and the protocol refers…
Tue, Jul 18, 2023
Will the NGAL (human) ELISA kit also work with species other than human?
This kit has been solely developed and validated for human samples. Please note however that Enzo has several other NGAL ELISA kits…
Tue, Jul 18, 2023
How should samples be stored after preparation for the NGAL (human) ELISA kit?
Samples should be aliquoted and stored at +2-8 °C for immediate analysis, or frozen at -20 °C for analysis within…
Tue, Jul 18, 2023