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Is the ISH/IHC Peroxidase block solution provided in the MULTIVIEW® PLUS IHC kit (ENZ-KIT181) the same as the IHC tissue primer (ADI-950-234)?
The peroxidase blocker solution is functionally the same as the IHC tissue primer but different in its formulation. The IHC tissue…
Tue, Jul 18, 2023
I plan to perform multiple IHC staining using your MULTIVIEW® PLUS IHC kit. What are your recommendations in case the targets co-localize?
In IHC, designing the experiment correctly is very critical. In the ideal situation, you will label unique proteins or antigens…
Tue, Jul 18, 2023
How can cross-reaction of detection reagents with primary antibodies be avoided using the MULTIVIEW® PLUS kit (ENZ-KIT181)?
Regarding the MULTIVIEW® PLUS (mouse-HRP/mouse-AP) IHC kit (Brown/Green), sufficient washes between antibody, detection, and chromogen steps (3 x 1 minute…
Tue, Jul 18, 2023
Will the HO-1 (h) ELISA cross react with HO-2 and HO-3?
This assay is specific for human HO-1 and will not cross react with human HO-2 and HO-3, the other known…
Tue, Jul 18, 2023
What is the nature of the recombinant HO-1 standard provided in the kit ADI-EKS-800, HO-1 (human), ELISA kit?
The standard is a recombinant human HO-1 (UniProt ID: P09601) expressed in E.coli.
Tue, Jul 18, 2023
How should I handle and store cell lysates if I cannot assay them immediately?
In order to prevent HO-1 degradation it is important to add protease inhibitors in the lysis buffer just prior to…
Tue, Jul 18, 2023
What are your recommendations as for mounting media and post-processing for your HIGHDEF® chromogens?
All our chromogens should be used preferentially with an aqueous-based mounting medium (e.g. ADI-950-261) for a better resolution and a…
Tue, Jul 18, 2023
Does the product ADI-EKS-800, HO-1 (human), ELISA kit also work for human plasma and serum?
The kit has been validated for the measurement of intracellular HO-1 in cell lysates and tissue extracts and the protocol refers…
Tue, Jul 18, 2023
Will the NGAL (human) ELISA kit also work with species other than human?
This kit has been solely developed and validated for human samples. Please note however that Enzo has several other NGAL ELISA kits…
Tue, Jul 18, 2023
Will my assay results vary depending on the sample type I am going to analyze with the NGAL (human) ELISA kit?
Analyses of samples of serum, EDTA plasma, citrate plasma, heparin plasma or urine showed no significant differences in analytical recovery,…
Tue, Jul 18, 2023