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Protein Analysis
High Background
How was the plate washed? It is important that the plate is washed thoroughly. If plate washing is troublesome, a…
Tue, Jul 18, 2023
Edge Effects
Where was the plate incubated? Often times the conditions for ambient incubations can be less than ideal. If there is…
Tue, Jul 18, 2023
Is extraction of vasopressin necessary prior to using the Vasopressin ELISA kit?
Enzo Vasopressin ELISA kit does require extraction of vasopressin from all sample types for accurate determination.
Tue, Jul 18, 2023
Does the Vasopressin ELISA kit work with saliva samples?
We have not tested saliva in-house with this kit. However, several researchers have shared with us the protocol described below for using that…
Tue, Jul 18, 2023
What is the recommended assay concentration range for KillerTRAIL™ and SuperKillerTRAIL™?
The recommended working dilution range for KillerTRAIL™ is 10-100 ng/ml, and 2-100 ng/ml for SuperKillerTRAIL™. However, adjustment may be necessary for the end-user’s specific…
Tue, Jul 18, 2023
What are the differences between TRAIL, KillerTRAIL™ and SuperKillerTRAIL™?
To induce apoptosis, TRAIL needs to form multimers, showing increasing activity with increasing multimerization levels. Multimerization of TRAIL can be…
Tue, Jul 18, 2023
Do oxytocin kits work on other sample types other than culture supernatants, milk, plasma or serum?
Cited sample types include cerebral spinal fluids (CSF), feces, plasma, saliva, serum, tissue, and urine.
Tue, Jul 18, 2023
Is there an alternative protocol for extraction of oxytocin in serum or plasma samples prior to using the Oxytocin ELISA kit?
The procedure described below is an alternative protocol for extraction of oxytocin from aqueous solutions. 1. Add two volumes of ice…
Tue, Jul 18, 2023
How much sample do I need to use and what are the suggested dilutions for the Oxytocin ELISA kit?
The amount of sample required and the dilutions needed for the assay depend upon the level of oxytocin in the…
Tue, Jul 18, 2023
I am working with rabbit sera. Can I use the Vasopressin ELISA kit for detecting vasopressin in this sample?
Problems may arise when working with rabbit sera because the kit relies on the use of an anti-rabbit IgG. This…
Tue, Jul 18, 2023