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Protein Analysis
Poor Standard Curve
What was used as the standard diluent? Diluents other than the supplied assay buffer may contain interfering substances that can…
Tue, Jul 18, 2023
Edge Effects
Where was the plate incubated? Often times the conditions for ambient incubations can be less than ideal. If there is…
Tue, Jul 18, 2023
Does the Vasopressin ELISA kit work with saliva samples?
We have not tested saliva in-house with this kit. However, several researchers have shared with us the protocol described below for using that…
Tue, Jul 18, 2023
Is extraction of vasopressin necessary prior to using the Vasopressin ELISA kit?
Enzo Vasopressin ELISA kit does require extraction of vasopressin from all sample types for accurate determination.
Tue, Jul 18, 2023
Do vasopressin kits work on other sample types other than culture supernatants?
Cited sample types include cerebrospinal fluid (CSF), culture supernatants, feces, plasma, saliva, serum, tissue lysate, and urine. In general, the…
Tue, Jul 18, 2023
What type of column can be used for oxytocin extraction?
Solid phase extraction (SPE) can be performed using reverse phase C18 columns containing 200 mg of sorbent and a volume capacity…
Tue, Jul 18, 2023
Is extraction of oxytocin necessary prior to using the Oxytcin ELISA kit?
Oxytocin ELISA kit does require extraction from serum and plasma. This extraction is required because of matrix interference with serum…
Tue, Jul 18, 2023
Does the Oxytocin ELISA kit work with saliva samples?
Although saliva was not a sample type tested during the development of this kit, there has been significant interest in…
Tue, Jul 18, 2023
What is the recommended assay concentration range for KillerTRAIL™ and SuperKillerTRAIL™?
The recommended working dilution range for KillerTRAIL™ is 10-100 ng/ml, and 2-100 ng/ml for SuperKillerTRAIL™. However, adjustment may be necessary for the end-user’s specific…
Tue, Jul 18, 2023
What are the differences between TRAIL, KillerTRAIL™ and SuperKillerTRAIL™?
To induce apoptosis, TRAIL needs to form multimers, showing increasing activity with increasing multimerization levels. Multimerization of TRAIL can be…
Tue, Jul 18, 2023