Indirect Immunoassays
The enzyme-linked immunosorbent assay (ELISA Kits), also known as an indirect immunoassay, is a third type of immunoassay commonly used…
Tue, Jul 18, 2023
Fluorescence Polarization Immunoassays
Fluorescence polarization immunoassays (FPIA) are quick and efficient homogeneous mix-and-read assays. The principle of the FPIA is similar to that…
Tue, Jul 18, 2023
Immunometric Assays
Immunometric assays, also known as sandwich assays, use two antibodies specific to the antigen to capture or “sandwich” antigen in…
Tue, Jul 18, 2023
Competitive Immunoassays
In competitive ELISA Assays, the antigen in a sample competes for limited antibody binding sites with antigen conjugated to a…
Tue, Jul 18, 2023
Immunoassay Basics
Understanding the basic principles of immunoassays is easy. The essential components of antibody-based immunoassay systems are threefold: an antigen that…
Tue, Jul 18, 2023
Detection Methods for Immunoassays
The immunological part of an immunoassay ends typically with either a wash or separation step. In these steps, any unbound…
Tue, Jul 18, 2023
When is it appropriate to use a CLIA instead of an EIA?
A CLIA, or chemiluminescent immunoassay, is more sensitive than an EIA (enzyme immunoassay) and is used when samples contain very…
Tue, Jul 18, 2023
Why are my ODs different than those shown in the instruction manual?
It is normal to see a small variation in OD values between experiments. OD values can be affected by small…
Tue, Jul 18, 2023
Can you explain why I am seeing inconsistent standard curves run in culture media?
Often times, the serum supplemented into media can be problematic. In order to minimize this problem it is very important…
Tue, Jul 18, 2023
How do I know if I need to dilute or extract my samples?
If your samples contain analyte levels within the dynamic range of the assay at the minimum recommended dilution for your…
Tue, Jul 18, 2023
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