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Protein Analysis
Indirect Immunoassays
The enzyme-linked immunosorbent assay (ELISA Kits), also known as an indirect immunoassay, is a third type of immunoassay commonly used…
Tue, Jul 18, 2023
Fluorescence Polarization Immunoassays
Fluorescence polarization immunoassays (FPIA) are quick and efficient homogeneous mix-and-read assays. The principle of the FPIA is similar to that…
Tue, Jul 18, 2023
Immunometric Assays
Immunometric assays, also known as sandwich assays, use two antibodies specific to the antigen to capture or “sandwich” antigen in…
Tue, Jul 18, 2023
Competitive Immunoassays
In competitive ELISA Assays, the antigen in a sample competes for limited antibody binding sites with antigen conjugated to a…
Tue, Jul 18, 2023
Immunoassay Basics
Understanding the basic principles of immunoassays is easy. The essential components of antibody-based immunoassay systems are threefold: an antigen that…
Tue, Jul 18, 2023
When is it appropriate to use a CLIA instead of an EIA?
A CLIA, or chemiluminescent immunoassay, is more sensitive than an EIA (enzyme immunoassay) and is used when samples contain very…
Tue, Jul 18, 2023
Can you explain why I am seeing inconsistent standard curves run in culture media?
Often times, the serum supplemented into media can be problematic. In order to minimize this problem it is very important…
Tue, Jul 18, 2023
What is the best way to analyze my data? What is 4-PL curve-fitting?
For the most accurate results we recommend that 4 parameter logistic (4-PL) curve fitting software be used. The object of…
Tue, Jul 18, 2023
What kind of instrumentation is required to read a CLIA assay?
A plate luminometer is necessary to read the RLU (relative light units) generated by these assays. It is not necessary…
Tue, Jul 18, 2023
Detection Methods for Immunoassays
The immunological part of an immunoassay ends typically with either a wash or separation step. In these steps, any unbound…
Tue, Jul 18, 2023