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Can cells be fixed and permeabilized after staining with Nuclear-ID® cell viability reagents (ENZ-53004, ENZ-53005, ENZ-53006)?
Cells can be fixed and permeabilized after staining with the NUCLEAR-ID® cell viability reagents (ENZ-53004, ENZ-53005, ENZ-53006). For best results,…
Tue, Jul 18, 2023
What is the consistency of the Antifade mounting medium hardset (ENZ-53002, ENZ-53003)?
The antifade mounting medium hardset (ENZ-53002, ENZ-53003) is thick and viscous in consistency similar to glycerol.
Tue, Jul 18, 2023
Can antifade mounting medium hardset (ENZ-53002, ENZ-53003) be used with fluorescein and rhodamine?
Yes, the antifade mounting medium hardeset (ENZ-53002, ENZ-53003) is recommended for use on fiwed cells and tissus that have been…
Tue, Jul 18, 2023
What is the application of the antifade mounting medium hardset (ENZ-53002, ENZ-53003) ?
The Antifade mounting medium hardeset can be used as a mouning medium fo fixed cells and tissues, stained with fluorescent…
Tue, Jul 18, 2023
What is the reason for the use of dye efflux inhibitor in the FLUOFORTE® Calcium assay kit (ENZ-51016, ENZ-51017, ENZ-51020)? Should this be used with all cell lines or is there an exception?
The use of the dye efflux inhibitor in the FLUOFORTE® Calcium assay kit (ENZ-51016, ENZ-51017, ENZ-51020) can help preventing the…
Tue, Jul 18, 2023
How long is the CYTO-ID® long-term cell tracer dye (ENZ-51036, ENZ-51037) retained in the cell and what cells were used to validate the assay?
The dye is well retained by cells for up to 96-120 hours after loading depending on how fast the cells…
Tue, Jul 18, 2023
What is the structure of the FluoForte dye and how does the dye detect Calcium levels in the cell?
The fluorogenic calcium-binding dye is a proprietary acetoxymethyl (AM) ester, which is cell-permeable. Once the dye enters the cells, it…
Tue, Jul 18, 2023
Is it possible to detect changes in calcium level in cells nuclei following specific treatment with the FLUOFORTE® Calcium assay kits (ENZ-51016, ENZ-51017, ENZ-51020)?
Detecting the calcium level in cells nuclei would require optimization. FLUOFORTE® Calcium Assay Kits (ENZ-51016, ENZ-51017, ENZ-51020) have been benchmarked for…
Tue, Jul 18, 2023
Is the CYTO-ID® Long Term Tracer Dye (ENZ-51036, ENZ-51037) toxic to cells?
Our CYTO-ID long-term cell tracer kits (ENZ-51036, ENZ-51037) use non-covalent cell labeling technology to stably incorporate the fluorescent dye containing…
Tue, Jul 18, 2023
Can the CYTO-ID® dye (ENZ-51036, ENZ-51037) be injected into mice for in vivo studies or does it need to be loaded into cultured cells?
CYTO-ID® long-term cell tracer kits (ENZ-51036, ENZ-51037) have not been tested for in vivo studies. The CYTO-ID dye would be difficult…
Tue, Jul 18, 2023