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Can the FluoForte® Calcium assay kit be used to monitor Ca2+ flux in nicotinic acetylcholine receptors on Flexstation. Can the FluoForte dye monitor Ca2+ in our model system given that FluoForte dye has been validated only in GPCR signaling path
While no specific references are available for application of FluoForte to assay nicotinic receptor, there are numerous publications regarding the…
Tue, Jul 18, 2023
What is the suggested remedy to minimize signal in the FITC emission filter while co-staining other proteins?
The excitation and emission spectra of the dye is provided on page 12 of the kit manual. The spectrum indicates…
Tue, Jul 18, 2023
Can the Proteostat kit be used to study protein aggregation with gram negative bacteria?
LPS in outer membrane of gram negative bacteria can be problematic. The permeabilizing buffer described in the manual, would remove…
Tue, Jul 18, 2023
Can the Proteostat kit be used to study protein aggregation with prokaryote samples?
The dye should work with prokaryotes if membrane fractions are separated. If the membrane fraction is not removed, the dye…
Tue, Jul 18, 2023
Is there a step in the protocol of ENZ-51035 at which it is possible to stop and store the samples at 40 C overnight and the protocols can be completed the next day?
It is advisable to stop the protocol if desired before loading the dye. The intensity of the dye decreases over…
Tue, Jul 18, 2023
What is a good positive control to study protein aggregation in cells?
MG132 treated cells would serve as positive control to determine background noise compared to signal along with untreated negative control.
Tue, Jul 18, 2023
Can I measure the levels of 8-OHdG in cell or tissue extracts?
The order of events happening in the cell is that the damaged DNA is modified, halting further transcription. Repair of…
Tue, Jul 18, 2023
Can I use plasma samples with ADI-EKS-350?
Plasma samples were not specifically tested in-house with this assay but there is no reason to believe it would not…
Tue, Jul 18, 2023
Do you have any literature references for normal human 8-OHdG levels using ADI-EKS-350?
Unfortunately, there is no real “gold standard” for comparing normal or expected levels of 8-OHdG. The latter is a rather…
Tue, Jul 18, 2023
I am planning to quantify 8-OHdG in cancer patient samples using ADI-EKS-350. This kit can be used to test urine, serum, and saliva samples. Which sample should I use?
Urine is usually the sample of choice for 8-OHdG when looking at DNA damage; however, which sample may be best…
Tue, Jul 18, 2023