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ELISA Immunoassays
Will the LTB4 ELISA kit work with serum samples?
This kit has not been validated internally for serum. Sample types that have been validated internally are ETDA plasma, cultured media, saliva…
Tue, Jul 18, 2023
Which antibodies are used in your HSP70 ELISA kits (ADI-EKS-700B, ADI-EKS-715, and ENZ-KIT-101)?
This information is considered to be proprietary and cannot be disclosed. The antibody recognizes an internal epitope of HSP70. The…
Tue, Jul 18, 2023
How should I handle and store cell lysates if I cannot assay them immediately?
In order to prevent HO-1 degradation it is important to add protease inhibitors in the lysis buffer just prior to…
Tue, Jul 18, 2023
What is the nature of the recombinant HO-1 standard provided in the kit ADI-EKS-800, HO-1 (human), ELISA kit?
The standard is a recombinant human HO-1 (UniProt ID: P09601) expressed in E.coli.
Tue, Jul 18, 2023
Will the HO-1 (h) ELISA cross react with HO-2 and HO-3?
This assay is specific for human HO-1 and will not cross react with human HO-2 and HO-3, the other known…
Tue, Jul 18, 2023
I have always seen the TMB Substrate Solution as a clear solution, but today the solution had significant green-blue tinge. What might have caused this color change?
The color tinge in the TMB substrate is due to contamination by the conjugate. To avoid contamination it is important…
Tue, Jul 18, 2023
Does the product ADI-EKS-800, HO-1 (human), ELISA kit also work for human plasma and serum?
The kit has been validated for the measurement of intracellular HO-1 in cell lysates and tissue extracts and the protocol refers…
Tue, Jul 18, 2023
Will the NGAL (human) ELISA kit also work with species other than human?
This kit has been solely developed and validated for human samples. Please note however that Enzo has several other NGAL ELISA kits…
Tue, Jul 18, 2023
How should urine samples be prepared for use with the NGAL (human) ELISA kit?
Urine preparation usually only requires centrifugation if there is inflammation or infection of the urinary tract (to remove polymorphonuclear neutrophils…
Tue, Jul 18, 2023
How should plasma samples be prepared for use with the NGAL (human) ELISA kit?
Collect whole blood into commercially available anticoagulant-treated tubes e.g., EDTA-treated (lavender tops) or citrate-treated (light blue tops). Heparinized tubes (green…
Tue, Jul 18, 2023