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Cellular Analysis
Under what conditions were the cells subjected to create hypoxic conditions with the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042? Were the cells grown under 95% N2 5 % CO2 ?
When validating the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, cells were grown in 95% nitrogen and also validated with either…
Tue, Jul 18, 2023
Can ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, be used for microplate assay format?
ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, has not been validated in microplate format. The kit is validated for fluorescence microscopy and…
Tue, Jul 18, 2023
Is the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, specific for live cell imaging? Can cells be fixed after staining?
Yes, the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, is validated on living cells only and tt would not be possible…
Tue, Jul 18, 2023
How long does the fluorescence last post-staining with the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042?
Storing the samples stained with the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, protected from exposure to direct light would help…
Tue, Jul 18, 2023
Does the Hypoxia Red Detection Reagent provided with ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, retain the red stain during oxygenation?
The hypoxia dye in the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042, does not fade during oxygenation.
Tue, Jul 18, 2023
What is the half-life of Hypoxia Red Detection Reagent and Oxidative Stress Detection Reagent in the ROS-ID® Hypoxia/Oxidative stress detection kit, ENZ-51042?
We have not determined the half-life of the dyes provided with the ROS-ID® Hypoxia/Oxidative stress detection kit (ENZ-51042) but both…
Tue, Jul 18, 2023
Is the peptide substrate coated on the microtiter plate present in the ADI-EKS-390A kit specific for PKA?
No, the peptide substrate in the PKA kinase activity kit (ADI-EKS-390A) is not specific to PKA and therefore it can…
Tue, Jul 18, 2023
Can DAPI be used as counterstaining for the nucleus with the nucleolar green detection kit Nucleolar-ID® Green detection kit for microscopy ENZ-51009 ?
Hoechst 33342 has been shown to work well. DAPI and Hoechst have similar spectral characteristics and either of them should work…
Tue, Jul 18, 2023
Do the EFLUXX-ID® multidrug resistance assay kits (ENZ-51029, ENZ-51030) require a reading by FACS or could a fluorescence reader such as Envision reader be used?
The the EFLUXX-ID® multidrug resistance assay kits (ENZ-51029, ENZ-51030) have been validated for Flow Cytometry and Microscopy. This does not…
Tue, Jul 18, 2023
Can different colored dyes be used to measure a different parameter with eThe eFluxx-ID® multidrug resistance assay kits (ENZ-51029, ENZ-51030)?
It is possible to use a dye which would not have spectral overlap with the EFLUXX-ID® dyes (ENZ-51029, ENZ-51030) and…
Tue, Jul 18, 2023