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Cellular Analysis
Antifade mounting medium hardset (ENZ-53002, ENZ-53003) appears to be clumped. What is the recommendation to get this in liquid form?
Antifade mounting medium hardset (ENZ-53002, ENZ-53003) needs to be warmed at 37-42°C for about 20 minutes, then vortexedt, if clumps are…
Tue, Jul 18, 2023
What is the consistency of the Antifade mounting medium hardset (ENZ-53002, ENZ-53003)?
The antifade mounting medium hardset (ENZ-53002, ENZ-53003) is thick and viscous in consistency similar to glycerol.
Tue, Jul 18, 2023
What is the mechanism of action for LYSO-ID® detection dyes (ENZ-51034, ENZ-51015, ENZ-51005)?
LYSO-ID® detection dyes provided with references ENZ-51034, ENZ-51015, ENZ-51005 are proprietary cationic amphiphilic tracers (CAT) that rapidly and selectively stains acidic…
Tue, Jul 18, 2023
What is the chemical identity of the LYSO-ID® dyes (ENZ-51034, ENZ-51015, ENZ-51005)?
The precise chemical identity of the LYSO-ID® detection dyes provided with references ENZ-51034, ENZ-51015, ENZ-51005 is proprietary. These are a cell…
Tue, Jul 18, 2023
Can cells be fixed and permeabilized after staining with Nuclear-ID® cell viability reagents (ENZ-53004, ENZ-53005, ENZ-53006)?
Cells can be fixed and permeabilized after staining with the NUCLEAR-ID® cell viability reagents (ENZ-53004, ENZ-53005, ENZ-53006). For best results,…
Tue, Jul 18, 2023
How long is the NUCLEAR-ID® red dye (ENZ-52406) retained by the cells and is the dye toxic to the cells?
NUCLEAR-ID® red DNA stain ( ENZ-52406) is fairly stable. We do not have specifics on how long the dye would be…
Tue, Jul 18, 2023
Do you offer different colored dyes with the same binding capacity to dsDNA?
We offer red, blue or green fluorescent dyes suitable for dsDNA binding, as a stand alone or as part of…
Tue, Jul 18, 2023
How specific is the NUCLEAR-ID® red dye (ENZ-52406) for dsDNA? What is the binding capacity of the dye to ssRNA, ssDNA and DNA:RNA Hybrids?
The dye stains dsDNA. Data is not available for binding capacity to ssRNA, ssDNA and DNA:RNA hybrids.
Tue, Jul 18, 2023
What is the recommended exposure setting for NUCLEAR-ID® red stain (ENZ-52406)?
The exposure depends on the microscope and filters. With a 50% neutral density filter, 1 second exposure is a good starting point.
Tue, Jul 18, 2023
Is the Red NUCLEAR-ID® dye in the NUCLEAR-ID® Blue/Red cell viability reagent provided with reference ENZ-53005 the same dye as NUCLEAR-ID Red for cell cycle analysis provided with reference ENZ-51008?
No, these are two different dyes.
Tue, Jul 18, 2023