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Knowledge Base
Can samples be frozen before clean up?
No. Samples should be stored at +4 °C after the addition of the stop buffer. They should not be frozen,…
Tue, Jul 18, 2023
How should the DNA be purified after labeling with Enzo’s CGH labeling kits?
Enzo's PCR & Gel Clean-up columns (ENZ-GEN100) are strongly recommended in this step. Also, Qiagen's QIAquick purification columns and buffers…
Tue, Jul 18, 2023
The datasheet for my compound states to add BSA or serum to the aqueoussolution before attempting to dilute my stock solution. Why is this suggested?
BSA and other serum albumins are natural carrier proteins for hydrophobic small molecules such as hormones and fatty acids. They…
Tue, Jul 18, 2023
The datasheet for my compound states that it will dissolve at a certain concentration, but I still see solid material. What should I do?
In most cases the speed at which a crystalline solid will dissolve is dependent upon three main factors, the affinity…
Tue, Jul 18, 2023
How should I handle waxy or low-melting point solids?
Compounds with relatively low melting points will often appear to be either waxy or sticky solids. These materials are difficult…
Tue, Jul 18, 2023
How should I handle hygroscopic compounds?
Hygroscopic compounds will absorb water from the atmosphere and should be stored dessicated and protected from exposure to the air…
Tue, Jul 18, 2023
Does the Vasopressin ELISA kit work with saliva samples?
We have not tested saliva in-house with this kit. However, several researchers have shared with us the protocol described below for using that…
Tue, Jul 18, 2023
Is extraction of vasopressin necessary prior to using the Vasopressin ELISA kit?
Enzo Vasopressin ELISA kit does require extraction of vasopressin from all sample types for accurate determination.
Tue, Jul 18, 2023
How should I dissolve a peptide and store aliquots?
Peptides should be stored at -20 °C for maximum stability. Because peptides are often hygroscopic, and water content can significantly…
Tue, Jul 18, 2023
My stock solution was initially clear, but when I removed it from the freezer to use it a second time, a precipitate formed. What should I do?
The process of a solution freezing and thawing can sometimes cause chemicals to crystallize or precipitate out of solution. This…
Tue, Jul 18, 2023