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What are the advantages of the Cyto-ID® autophagy dye relative to monodansyl cadaverine (MDC)?
MDC requires 365 nm UV illumination and thus, is not compatible with 488 nm excitation sources commonly implemented in flow…
Tue, Jul 18, 2023
What are the advantages of the Cyto-ID® Autophagy Detection kit relative to GFP-LC3 transfected cells?
The most significant advantage for Cyto-ID® Autophagy Detection kit is that it's a no-transfection assay. Transfections are transient and there…
Tue, Jul 18, 2023
How stable is the Cyto-ID® Green autophagy dye?
Fluorescent dyes are subject to photobleaching, but this dye is more robust than dyes like fluorescein. Standard handling precautions should…
Tue, Jul 18, 2023
Are the cells fixable after staining with the autophagy kit?
Yes, a protocol for fixing cells is provided in the product manual. Permeabilization of the cellular membrane with detergents, however,…
Tue, Jul 18, 2023
Can the Cyto-ID® green detection dye be used in conjunction with another dye to differentiate between endosomes and amphisomes?
The Cyto-ID® Red long-term tracer dye, ENZ-51037- inserts into the plasma membrane and later is internalized by endocytosis. Cyto-ID® Red…
Tue, Jul 18, 2023
Bright yellow array with reduced magnitude of expected changes
This can be due to insufficient Cot-1 DNA, blocking agents or hybridization and washing conditions that are not stringent enough.
Tue, Jul 18, 2023
Good DLRs in the CGH analytics QC report, but some oligos show whiskering or significant deviation from the expected log2 ratio
We suggest keeping the Cot-1 DNA at 50 µg regardless of the hybridization volume.
Tue, Jul 18, 2023
Precipitate is seen in the 10X assay buffer
Precipitate forms at low temperatures. Allow solution to warm to room temperature or 37°C.
Tue, Jul 18, 2023
*General Information
The solubility of small molecule products, especially those that are hydrophobic can sometimes be a limiting factor in experimental design.…
Tue, Jul 18, 2023
Now that i’ve opened my product and used it for the first time, how should i store the leftover material?
Recommendations for storage after initial use may be provided on the product datasheet or certificate of analysis. These vary depending upon…
Tue, Jul 18, 2023