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Knowledge Base
For the ROS/RNS detection kit, ENZ-51001, can samples be stored after the final wash buffer step in the protocol, and do the microscopy at a later date?
No, this is a vital stain. Live cells must be read immediately per the manual.
Tue, Jul 18, 2023
Poor Standard Curve
What was used as the standard diluent? Diluents other than the supplied assay buffer may contain interfering substances that can…
Tue, Jul 18, 2023
How long can the Cyto-ID® green detection reagent be left incubating with live cells (mins/hours/days)?
We do not recommend to incubate cells with Cyto-ID® green detection reagent for longer that 30 minutes at 37°C. In…
Tue, Jul 18, 2023
Can GFP-Certified™ Apoptosis/Necrosis Detection Kit, ENZ-51002, and Cyto-ID® Autophagy Detection Kit, ENZ-51031, be combined to perform triple staining simultaneously detecting apoptotic, necrotic and autophagic cells ?
GFP-Certified™ Apoptosis/Necrosis Detection Kit, ENZ-51002, and Cyto-ID®Autophagy Detection Kit, ENZ-51031, cannot be used in combination because of spectral overlap between…
Tue, Jul 18, 2023
Using this kit, is it necessary to wash the cells after staining?
No, the kit does not require a wash step.
Tue, Jul 18, 2023
Edge Effects
Where was the plate incubated? Often times the conditions for ambient incubations can be less than ideal. If there is…
Tue, Jul 18, 2023
Poor Precision
Were the wells washed properly? All wells receive the same treatment during the wash step. If some are washed less…
Tue, Jul 18, 2023
Drift
Were reagents brought to room temperature prior to use? If the reagents are not at a constant temperature prior to…
Tue, Jul 18, 2023
Weak Color Development
Was substrate added at the correct point in the assay? See the assay procedure provided in the instruction manual. Were…
Tue, Jul 18, 2023
What is the Kd and quantum yield of FluoForte® Calcium Assay kit?
Kd is estimated to be 389 nM (22°C, pH 7.0 –7.5). Maximum quantum yield is 0.18 at pH 7.2.
Tue, Jul 18, 2023