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When using an eicosanoid ELISA kit, how much indomethacin should I use in my samples?
You should follow the suggestion in the product specific manual, and add up to 10µg per ml of liquid sample.…
Tue, Jul 18, 2023
If I have 2N NaOH hydrolyzed samples for an 8-iso-PGF2a determination, do I need to extract these samples?
No, the Direct 8-iso-PGF2a kit is specifically designed to help you obtain results as quickly and easily as possible. The…
Tue, Jul 18, 2023
What is the difference between the 8-iso-PGF<sub><span style="font-size: 10pt;">2a</span></sub> ELISA kit and the Direct 8-iso-PGF<sub><span style="font-size: 10pt;">2a</span></sub> ELISA kit?
The regular 8-iso-PGF2a kit is designed to measure free isoprostane in samples. Direct 8-iso-PGF2a measures total isoprostane levels, both free…
Tue, Jul 18, 2023
Can I measure urinary steroid levels in Enzo’s hormone ELISA kits?
Steroids are relatively insoluble and must be altered by being conjugated to glucoronic acid for elimination. The antibodies in our…
Tue, Jul 18, 2023
I am seeing much higher than expected nitrate levels. What could be the problem?
Many times the water source in the lab is the culprit. The water is only as pure as the last…
Tue, Jul 18, 2023
It says in the insert that samples containing high levels of NADPH need to be diluted sufficiently. How can I determine the NADPH levels and dilution prior to running the Nitric Oxide detection assay?
We have found that 0.5-1mM NADPH will inhibit the Griess color reaction slightly. If this amount of NADPH is present…
Tue, Jul 18, 2023
What type of 10,000 MWCO filter is best to use prior to using the Nitric Oxide detection kits?
The user has a choice between a spin-filter, a syringe-type filter, or a 96-well filtration plate format. Generally, a filter…
Tue, Jul 18, 2023
Why do I have to filter my samples through a 10,000 MWCO filter prior to using the Nitric Oxide detection kits?
In order to get the best sensitivity, samples need to be filtered to remove or reduce interfering substances. Using a…
Tue, Jul 18, 2023
Can I run cell lysates with the Nitric Oxide detection kits?
Yes. First remove the media from the cells. Pellet them and wash with PBS before lysing the cells in the…
Tue, Jul 18, 2023
My cell culture samples are in DMEM. Will this interfere with the Nitric Oxide assay?
Yes. The nitrate salt used in this particular media will cause interference. It is important to check the published media…
Tue, Jul 18, 2023