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What could be the reason when no red fluorescence is detected in a sample containing both live and dead cells if the blue DAPI works well and the protocols were followed as recommended?
The filter settings should be checked to determine that they are optimal. Since the blue signal is stronger than the…
Tue, Jul 18, 2023
Do you offer different colored dyes with the same binding capacity to dsDNA?
We offer red, blue or green fluorescent dyes suitable for dsDNA binding, as a stand alone or as part of…
Tue, Jul 18, 2023
Can calcium mobilization be observed over the course of 1 hour with FluoForte Calcium assay kit? How long can the cells retain the fluorescent dye?
Ligand binding to GPCR generates very fast kinetics for calcium release, with the mobilization complete in less than a minute…
Tue, Jul 18, 2023
Can the Proteostat kit be used to study protein aggregation with prokaryote samples?
The dye should work with prokaryotes if membrane fractions are separated. If the membrane fraction is not removed, the dye…
Tue, Jul 18, 2023
Is there a step in the protocol of ENZ-51035 at which it is possible to stop and store the samples at 40 C overnight and the protocols can be completed the next day?
It is advisable to stop the protocol if desired before loading the dye. The intensity of the dye decreases over…
Tue, Jul 18, 2023
Can I use plasma samples with ADI-EKS-350?
Plasma samples were not specifically tested in-house with this assay but there is no reason to believe it would not…
Tue, Jul 18, 2023
Do you have any literature references for normal human 8-OHdG levels using ADI-EKS-350?
Unfortunately, there is no real “gold standard” for comparing normal or expected levels of 8-OHdG. The latter is a rather…
Tue, Jul 18, 2023
I am planning to quantify 8-OHdG in cancer patient samples using ADI-EKS-350. This kit can be used to test urine, serum, and saliva samples. Which sample should I use?
Urine is usually the sample of choice for 8-OHdG when looking at DNA damage; however, which sample may be best…
Tue, Jul 18, 2023
What do I need to do if my antibody concentration is higher than the 50 µg/mL indicated in the manual for ADI-900-057?
If you have samples with an antibody concentration higher than 50 µg/mL, you just need to dilute these samples down to…
Tue, Jul 18, 2023
Can the cells be trypsinized to determine protein aggregation in cell based assay for testing drug responsiveness?
Trypsin can be used with ProteoStat® Aggresome detection kit. Following lysis, detergent can be added to permeabilize the cells. The…
Tue, Jul 18, 2023