Lab Essentials
AMPIVIEW® RNA probes
Enabling Your Projects 
GMP Services
Bulk Solutions
Research Travel Grant
Have You Published Using an Enzo Product?
Knowledge Base
Is it possible to detect changes in calcium level in cells nuclei following specific treatment with the FLUOFORTE® Calcium assay kits (ENZ-51016, ENZ-51017, ENZ-51020)?
Detecting the calcium level in cells nuclei would require optimization. FLUOFORTE® Calcium Assay Kits (ENZ-51016, ENZ-51017, ENZ-51020) have been benchmarked for…
Tue, Jul 18, 2023
What is the recommended exposure setting for NUCLEAR-ID® red stain (ENZ-52406)?
The exposure depends on the microscope and filters. With a 50% neutral density filter, 1 second exposure is a good starting point.
Tue, Jul 18, 2023
Can the cells be trypsinized to determine protein aggregation in cell based assay for testing drug responsiveness?
Trypsin can be used with ProteoStat® Aggresome detection kit. Following lysis, detergent can be added to permeabilize the cells. The…
Tue, Jul 18, 2023
Can cell lysates be used to determine protein aggregation with ProteoStat® Aggresome detection reagent?
Working with cell lysates would be challenging, because detergents can cause high background. It is possible to titrate the amount…
Tue, Jul 18, 2023
Would diluting the sample to a lower concentration (e.g. 0.5 mg/mL) change the percentage of aggregate protein?
The percent aggregate (the ratio of aggregate to total protein) will stay the same. However, the concentration of aggregated protein…
Tue, Jul 18, 2023
Can calcium mobilization be observed over the course of 1 hour with FluoForte Calcium assay kit? How long can the cells retain the fluorescent dye?
Ligand binding to GPCR generates very fast kinetics for calcium release, with the mobilization complete in less than a minute…
Tue, Jul 18, 2023
Can the Proteostat kit be used to study protein aggregation with prokaryote samples?
The dye should work with prokaryotes if membrane fractions are separated. If the membrane fraction is not removed, the dye…
Tue, Jul 18, 2023
Is there a step in the protocol of ENZ-51035 at which it is possible to stop and store the samples at 40 C overnight and the protocols can be completed the next day?
It is advisable to stop the protocol if desired before loading the dye. The intensity of the dye decreases over…
Tue, Jul 18, 2023
Can I use plasma samples with ADI-EKS-350?
Plasma samples were not specifically tested in-house with this assay but there is no reason to believe it would not…
Tue, Jul 18, 2023
Do you have any literature references for normal human 8-OHdG levels using ADI-EKS-350?
Unfortunately, there is no real “gold standard” for comparing normal or expected levels of 8-OHdG. The latter is a rather…
Tue, Jul 18, 2023