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Knowledge Base
What is the required fragment length of input DNA for labeling with Enzo’s CGH labeling kits?
Comparative genome hybridization (CGH) does not require DANN fragmentation prior to analysis and can be performed with genomic input DANN.…
Tue, Jul 18, 2023
Does genomic DNA need to be fragmented for labeling with Enzo’s CGH labeling kits?
Restriction digestion or fragmentation of the isolated genomic DNA is not required prior to labeling with our CYTAG® CGH (ENZ-42671)…
Tue, Jul 18, 2023
What is the starting concentration of DNA needed for labeling with the CGH labeling kit for oligo arrays?
We suggest using 0.25 – 2.5 µg of input genomic DNA in a maximum volume of 19 µl, hence a…
Tue, Jul 18, 2023
Are Enzo’s CGH labeling kits suitable for single nucleotide polymorphism (SNP) analysis?
Our CYTAG® TotalCGH Labeling Kit (ENZ-42674) is perfectly suited for CGH+SNP array analysis. Our CYTAG® CGH (ENZ-42671) and CYTAG® Super…
Tue, Jul 18, 2023
Is there an alternate stop buffer that can be used with Enzo’s CGH labeling kits?
An EDTA solution may be used to stop the reaction prior to purification instead of the supplied stop buffer.
Tue, Jul 18, 2023
Which reference DNA can be used with Enzo’s CGH labeling kits?
Every high quality intact genomic DNA of healthy individuals may be used as a reference. Human male (ENZ-GEN106) and female…
Tue, Jul 18, 2023
How should I determine DNA concentration before labeling with Enzo’s CGH labeling kits?
We recommend determining concentration and purity of the genomic DNA using the nucleic acid module of a NanoDrop UV-VIS Spectrophotometer…
Tue, Jul 18, 2023
How long can the cells be saved until analysis by flow cytometry when using the Cellular senescence live cell analysis assay (ENZ-KIT130)?
Analyzing the results within a few hours is recommended because this assay uses live cells. If the cells lyse, the dye…
Tue, Jul 18, 2023
Can ENZ-KIT130 be used with cells expressing GFP?
ENZ-KIT130 is not recommended for use on GFP-expressing cells because of the emission spectra overlap between GFP and the fluorogenic…
Tue, Jul 18, 2023
When using the Cellular senescence live cell analysis assay(ENZ-KIT130), how long does the fluorescence last?
The fluorescence will last for several hours to one day.
Tue, Jul 18, 2023