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Could you recommend a control compound Calcineurin inhibitor to use with the Calcineurin Phosphatase assay kit, BML-AK804?
In the kit insert for our Calcineurin Phosphatase assay kit, BML-AK804, we list several inhibitors that were screened with the…
Tue, Jul 18, 2023
Can the kit BML-AK804, Calcineurin Phosphatase assay kit be used for biological samples?
The product BML-AK804, Calcineurin Phosphatase assay kit is designed for inhibitor screening or kinetic assays using the recombinant Calcineurin enzyme…
Tue, Jul 18, 2023
How should I determine DNA concentration before labeling with Enzo’s CGH labeling kits?
We recommend determining concentration and purity of the genomic DNA using the nucleic acid module of a NanoDrop UV-VIS Spectrophotometer…
Tue, Jul 18, 2023
Enzo suggests using 50 – 100 µg of Cot-1 DNA per sample when hybridizing the DNA labeled with the CGH labeling kits to Agilent arrays, is that correct?
The suggested amount of Cot-1 DNA in the protocols is correct. However, there is no theoretical reason why Enzo's kits…
Tue, Jul 18, 2023
Does genomic DNA need to be fragmented for labeling with Enzo’s CGH labeling kits?
Restriction digestion or fragmentation of the isolated genomic DNA is not required prior to labeling with our CYTAG® CGH (ENZ-42671)…
Tue, Jul 18, 2023
How can the quality of DNA labeling by Enzo’s CGH labeling kits be analyzed?
We recommend using a NanoDrop UV-VIS Spectrophotometer in the microarray measurement mode to determine yield and dye incorporation. Use 1.5…
Tue, Jul 18, 2023
Are Enzo’s CGH labeling kits suitable for single nucleotide polymorphism (SNP) analysis?
Our CYTAG® TotalCGH Labeling Kit (ENZ-42674) is perfectly suited for CGH+SNP array analysis. Our CYTAG® CGH (ENZ-42671) and CYTAG® Super…
Tue, Jul 18, 2023
Immunohistochemistry protocol validated for these antibodies
Immunohistochemistry protocol validated for the antibodies described below: ADI-905-781, ADI-KAP-GP005, ADI-VAM-PT046, ADI-VAM-SV016, ADI-VAM-SV020, ADI-VAM-SV021, ADI-VAM-SV026, ADI-VAP-PT049, ADI-VAP-SV065, ADI-AAP-113, ADI-CSA-815, ADI-OSA-110,…
Tue, Jul 18, 2023
Which reference DNA can be used with Enzo’s CGH labeling kits?
Every high quality intact genomic DNA of healthy individuals may be used as a reference. Human male (ENZ-GEN106) and female…
Tue, Jul 18, 2023
Is there an alternate stop buffer that can be used with Enzo’s CGH labeling kits?
An EDTA solution may be used to stop the reaction prior to purification instead of the supplied stop buffer.
Tue, Jul 18, 2023