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In which cases do you recommend to digest the DNA prior to labeling with Enzo’s CGH labeling kits?
For CGH+SNP arrays, genomic DNA must be digested using a combination of the restriction enzymes AluI and RsaI. For CGH…
Tue, Jul 18, 2023
What is the optimal time for hybridization of DNA that was labeled with Enzo’s CGH labeling kits?
Please follow the recommendations of the CGH array manufacturer you are using.
Tue, Jul 18, 2023
What will be the effect of decreasing the labeling time with Enzo’s CGH labeling kits below the recommended 4 hours?
For input DNA of good quality and quantity, shorter labeling times down to 2 hours may be sufficient, but the…
Tue, Jul 18, 2023
How can I stop the LEADING LIGHT® Wnt reporter cells from clumping?
It is important to remove the Cell Growth Medium entirely before adding trypsin to the cells. Usually, clumping is caused…
Tue, Jul 18, 2023
Which instrument should I use with your LEADING LIGHT® Wnt reporter assay starter kit to measure Wnt activity?
A plate luminometer capable of reading glow chemiluminescence is required. The light emitted by our LEADING LIGHT® Wnt reporter cell…
Tue, Jul 18, 2023
Do I really need to add Stop Buffer after the labeling reaction with Enzo’s CGH labeling kits even though the enzyme is heat-inactivated?
During kit validation, a protocol utilizing a 4-hour labeling step and addition of Stop Buffer gave us the best results…
Tue, Jul 18, 2023
For the Protein A ELISA kit, ADI-900-057, would the readings be affected if the wells were to dry out when residual wash buffer is blotted out?
In general with any ELISA, including the Protein A ELISA kit (ADI-900-057), care should be taken not to allow the…
Tue, Jul 18, 2023
Can the BIOARRAY™ Terminal labeling kit (ENZ-42630) be used to label sequences of 100 nucleotides?
The BIOARRAYTM Terminal labeling kit kit was optimized with a 30-mer at 1 µg or 100 pmol, but a longer…
Tue, Jul 18, 2023
Is the CYTAG™ CGH Labeling kit also suitable for BAC arrays?
The CYTAG™ CGH Labeling kit was designed for the more demanding high density oligo arrays, but it also works well…
Tue, Jul 18, 2023
How long can Enzo’s CGH labeling kits be stored after first use?
Independent of the date of first use we can only guarantee for performance of Enzo's CGH labeling kits for up…
Tue, Jul 18, 2023