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Do I need to use the LEADING LIGHT® Wnt cell line medium pack?
The LEADING LIGHT® Wnt cell line medium pack (ENZ-60003) is made of a cell freezing medium, a cell growth medium…
Tue, Jul 18, 2023
Can you share the recipe of the Cell Growth Medium?
This information is considered to be proprietary and we cannot disclose the actual recipe. It contains additives and nutrients. These…
Tue, Jul 18, 2023
How long should I incubate my LEADING LIGHT® Wnt reporter cells after adding the luciferase substrate?
Signals should be read 10 minutes after adding the luciferase substrate with a multichannel pipette. The signal is stable enough…
Tue, Jul 18, 2023
Which instrument should I use with your LEADING LIGHT® Wnt reporter assay starter kit to measure Wnt activity?
A plate luminometer capable of reading glow chemiluminescence is required. The light emitted by our LEADING LIGHT® Wnt reporter cell…
Tue, Jul 18, 2023
How can I improve the intensity of the signal obtained with the LEADING LIGHT® Wnt reporter cells?
Conditions in terms of cell density, culture medium, and incubation have to be respected. It is very important that there…
Tue, Jul 18, 2023
How should the Cyanine dyes of Enzo’s CGH labeling kits be stored after thawing?
For up to ten reactions, they can directly be refrozen at -20°C in the dark. For more reactions, they should…
Tue, Jul 18, 2023
What are the molar extinction coefficients of the Cyanine 3 and Cyanine 5 dyes in Enzo’s CGH labeling kits?
Cyanine 3: 150,000 M cm-1; Cyanine 5: 250,000 M cm-1
Tue, Jul 18, 2023
What are the key advantages of Enzo’s CGH labeling kits in comparison to the kits Agilent offers?
All our CGH labeling kits outperform the kits offered by Agilent for labeling: They provide excellent DNA yields with superior…
Tue, Jul 18, 2023
How should Enzo’s CGH labeling kits be stored?
Upon receipt, all reagents of the kit should be stored at –20 °C.
Tue, Jul 18, 2023
How should Enzo’s CGH labeling kits be handled?
We recommend aliquoting the kit reagents upon first use into volumes convenient for your workflow, and to avoid repeated freezing…
Tue, Jul 18, 2023