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Can gene-specific primers be used for cDNA synthesis with BIOARRAY™ Single-round RNA amplification and biotin labeling system, ENZ-42420 and ENZ-42421?
RNA amplification with BIOARRAYTM Single-round RNA amplification and biotin labeling system requires the use of primers included in the kit.…
Tue, Jul 18, 2023
How can background staining be minimized with ORGANELLE-ID-RGB® III assay kit, ENZ-51032?
When using ORGANELLE-ID-RGB® III assay kit (ENZ-51032) the cell medium could be a problem. Staining can be improved by…
Tue, Jul 18, 2023
Does 17ß-Estradiol ELISA kit, ADI-900-008 cross react with Estradiol 3-benzoate?
Estradiol 3-benzoate has not been determined for cross reactivity with17ß-Estradiol ELISA kit (ADI-900-008). The cross relativities for a number of…
Tue, Jul 18, 2023
What is the likely explanation for lack of staining cells with NUCLEAR-ID® Red DNA stain, ENZ-52406 for FACS application, when 250-fold and 500-fold dilution and incubation at 37°C for 30 minutes were tried?
It is important to check cell density and to ensure that cell concentration is 1 x 105 to 5 x…
Tue, Jul 18, 2023
Liver lysate samples when probed with HO-1 polyclonal antibody, ADI-SPA-896, shows two bands on western blot. What is the possible explanation as to why two bands detected?
Western blot image displayed on product page with ADI-SPA-896 shows two bands being detected. One of them is full length…
Tue, Jul 18, 2023
Does ORGANELLE-ID-RGB® III assay kit, ENZ-51032, bind to a target protein in ER and Golgi compartment?
The Golgi-green dye in the ORGANELLE-ID-RGB® III assay kit (ENZ-51032) is a fluorescent lipid analog which is transported directly into…
Tue, Jul 18, 2023
How specific are colorimetric/fluorogenic substrates in Matrix Metalloproteinase (MMP) drug discovery kits?
Several MMPs cleave both BML-P277-9090 substrate (OMNIMMP™ RED fluorogenic substrate peptide), BML-P278-9090 substrate (MPP-3 Fluorogenic Substrate peptide) and colorimetric substrate…
Tue, Jul 18, 2023
27 ml Assay Buffer is provided in ADI-901-002, ADI-901-153A, ADI-901-173 and in several other 5 plate ELSIA kits. Would this amount of buffer be sufficient for all of the samples after preparing standards?
Assay buffer in 5x 96 plate kits (e.g. in ADI-901-002, ADI-901-153A, ADI-901-173) is provided as 10x Concentrate and this requires…
Tue, Jul 18, 2023
What is the recommended dilutions for plasma samples obtained from subjects treated with aspirin for TXB2 ELISA kit (ADI-900-002 and ADI-901-002)?
When the TBX2 ELISA kit (ADI-900-002 and ADI-901-002) was developed with pooled samples, 1:100 dilution worked. So, this is a…
Tue, Jul 18, 2023
Does Enzo Protein A ELISA kit, ADI-900-057, detect Protein A purified on MabSelect PrismA and MabSelect SuRe™ resins?
Protein A ELISA kit (ADI-900-057) detects ligand that bind to GE bound MabSelect SuRe™ resin. We have not determined the…
Tue, Jul 18, 2023